A study of experimental autoimmune encephalomyelitis in dogs as a disease model for canine necrotizing encephalitis

In the present study, the use of dogs with experimental autoimmune encephalomyelitis (EAE) as a disease model for necrotizing encephalitis (NE) was assessed. Twelve healthy dogs were included in this study. Canine forebrain tissues (8 g), including white and grey matter, were homogenized with 4 mL o...

Full description

Saved in:
Bibliographic Details
Published in:Journal of veterinary science (Suwŏn-si, Korea) 2015, 16(2), , pp.203-211
Main Authors: Moon, Jong-Hyun, Jung, Hae-Won, Lee, Hee-Chun, Jeon, Joon-Hyeok, Kim, Na-Hyun, Sur, Jung-Hyang, Ha, Jeongim, Jung, Dong-In
Format: Article
Language:English
Subjects:
Animals
Brain - pathology
Disease Models, Animal
Dog Diseases - immunology
Dogs
Encephalitis - immunology
Encephalitis - veterinary
Encephalomyelitis, Autoimmune, Experimental - immunology
Encephalomyelitis, Autoimmune, Experimental - veterinary
Female
Fluorescent Antibody Technique - veterinary
Immunization - veterinary
Immunohistochemistry - veterinary
Magnetic Resonance Imaging - veterinary
Male
Necrosis - immunology
Necrosis - veterinary
Original
수의학
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the present study, the use of dogs with experimental autoimmune encephalomyelitis (EAE) as a disease model for necrotizing encephalitis (NE) was assessed. Twelve healthy dogs were included in this study. Canine forebrain tissues (8 g), including white and grey matter, were homogenized with 4 mL of phosphate-buffered saline for 5 min in an ice bath. The suspension was emulsified with the same volume of Freund's complete adjuvant containing 1 mg/mL of killed Mycobacterium tuberculosis H37Ra. Under sedation, each dog was injected subcutaneously with canine brain homogenate at four sites: two in the inguinal and two in the axillary regions. A second injection (booster) was administered to all the dogs using the same procedure 7 days after the first injection. Clinical assessment, magnetic resonance imaging, cerebrospinal fluid analyses, necropsies, and histopathological and immunohistochemical examinations were performed for the dogs with EAE. Out of the 12 animals, seven (58%) developed clinically manifest EAE at various times after immunization. Characteristics of canine EAE models were very similar to canine NE, suggesting that canine EAE can be a disease model for NE in dogs.
ISSN:1229-845X
1976-555X
DOI:10.4142/jvs.2015.16.2.203