Enhancing mucosal immunity in mice by recombinant adenovirus expressing major epitopes of porcine circovirus-2 capsid protein delivered with cytosine-phosphate-guanosine oligodeoxynucleotides

• Published in: Journal of veterinary science (Suwŏn-si, Korea) 2014, 15(3), , pp.399-407
• Main Authors: Chang, Hong-Tao, He, Xiu-Yuan, Liu, Yu-Feng, Chen, Lu, Guo, Quan-Hai, Yu, Qiu-Ying, Zhao, Jun, Wang, Xin-Wei, Yang, Xia, Wang, Chuan-Qing
• Format: Article
• Language: English
• Subjects: Adenoviridae - genetics; Adenoviridae - immunology; Administration, Intranasal; Animals; Capsid Proteins - genetics; Capsid Proteins - immunology; Circoviridae Infections - immunology; Circovirus - genetics; Circovirus - immunology; Epitopes - genetics; Epitopes - immunology; Female; Immunity, Mucosal - immunology; Immunoglobulin A - blood; Immunoglobulin A - immunology; Immunoglobulin G - blood; Immunoglobulin G - immunology; Mice; Mice, Inbred BALB C; Oligodeoxyribonucleotides - genetics; Original; Vaccines, Synthetic - genetics; Vaccines, Synthetic - immunology; Viral Vaccines - administration & dosage; Viral Vaccines - genetics; Viral Vaccines - immunology; 수의학
• ISSN: 1229-845X; 1976-555X
• DOI: 10.4142/jvs.2014.15.3.399

A recombinant replication-defective adenovirus expressing the major epitopes of porcine circovirus-2 (PCV-2) capsid protein (rAd/Cap/518) was previously constructed and shown to induce mucosal immunity in mice following intranasal delivery. In the present study, immune responses induced by intranasal immunization with a combination of rAd/Cap/518 and cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODN) were evaluated in mice. The levels of PCV-2-specific IgG in serum and IgA in saliva, lung, and intestinal fluids were significantly higher in the group immunized with rAd/Cap/518 and CpG ODN than animals immunized with rAd/Cap/518 alone. The frequencies of IL-2-secreting CD4⁺ T cells and IFN-γ-producing CD8⁺ T cells were significantly higher in the combined immunization group than mice immunized with rAd/Cap/518 alone. The frequencies of CD3⁺, CD3⁺CD4⁺CD8⁻, and CD3⁺CD4⁻CD8⁺ T cells in the combined immunization group were similar to that treated with CpG ODN alone, but significantly higher than mice that did not receive CpG ODN. PCV-2 load after challenge in the combined immunization group was significantly lower than that in the phosphate-buffered saline placebo group and approximately 7-fold lower in the group treated with CpG ODN alone. These results indicate that rAd/Cap/518 combined with CpG ODN can enhance systemic and local mucosal immunity in mice, and represent a promising synergetic mucosal vaccine against PCV-2.