Determination of angiotensin I-converting enzyme activity in equine blood: lack of agreement between methods of analysis

Angiotensin-I converting enzyme (ACE) is a key regulator of blood pressure, electrolytes and fluid homeostasis through conversion of angiotensin I into angiotensin II. Recently, a genetic polymorphism of the ACE gene, which accounts for 47% of the variation of ACE activity in blood, has been advocat...

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Published in:Journal of veterinary science (Suwŏn-si, Korea) 2011, 12(1), , pp.21-25
Main Authors: Costa, Maria Fernanda de M, Carmona, Adriana K, Alves, Marcio F M, Ryan, Timothy M, Davies, Helen M, Anderson, Garry A, Slocombe, Ron F
Format: Article
Language:English
Subjects:
Animals
Enzyme Assays - methods
Female
Fluorometry - methods
Horses - blood
Horses - genetics
Horses - metabolism
Male
Oligopeptides - pharmacology
Original
Peptidyl-Dipeptidase A - blood
Peptidyl-Dipeptidase A - genetics
Peptidyl-Dipeptidase A - metabolism
Polymorphism, Genetic
Reference Values
Spectrophotometry - methods
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Summary:Angiotensin-I converting enzyme (ACE) is a key regulator of blood pressure, electrolytes and fluid homeostasis through conversion of angiotensin I into angiotensin II. Recently, a genetic polymorphism of the ACE gene, which accounts for 47% of the variation of ACE activity in blood, has been advocated as a biomarker of athletic aptitude. Different methods of analysis and determination of ACE activity in plasma have been used in human and equine research without a consensus of a "gold standard" method. Different methods have often been used interchangeably or cited as being comparable in the existing literature; however, the actual agreement between assays has not been investigated. Therefore, in this study, we evaluated the level of agreement between three different assays using equine plasma obtained from 29 horses. Two spectrophotometric assays using Furylacryloylphenylalanyl-glycyl-glycine as substrate and one fluorimetric assay utilizing o-aminobenzoic acid-FRK-(Dnp)P-OH were employed. The results revealed that the measurements from the different assays were not in agreement, indicating that the methods should not be used interchangeably for measurement of equine ACE activity. Rather, a single method of analysis should be adopted to achieve comparable results and critical appraisal of the literature is needed when attempting to compare results obtained from different assays.
ISSN:1229-845X
1976-555X
DOI:10.4142/jvs.2011.12.1.21