Identification of Arcanobacterium pyogenes isolated by post mortem examinations of a bearded dragon and a gecko by phenotypic and genotypic properties

• Published in: Journal of veterinary science (Suwŏn-si, Korea) 2010, 11(3), , pp.265-267
• Main Authors: Ulbegi-Mohyla, H., Justus-Liebig-Universitat Gieβen, Gieβen, Germany, Hijazin, M., Justus-Liebig-Universitat Gieβen, Gieβen, Germany, Alber, J., Justus-Liebig-Universitat Gieβen, Gieβen, Germany, Lammler, C., Justus-Liebig-Universitat Gieβen, Gieβen, Germany, Hassan, A. A., GD Animal Health Service, Department of Bacteriology and Parasitology, Deventer, The Netherlands, Abdulmawjood, A., Justus-Liebig-Universitat Gieβen, Gieβen, Germany, Prenger-Berninghoff, E., Justus-Liebig-Universitat Gieβen, Gieβen, Germany, Weiß, R, Justus-Liebig-Universitat Gieβen, Gieβen, Germany, Zxchock, M., Landesbetrieb Hessisches Landeslabor, Gieβen, Germany
• Format: Article
• Language: English
• Subjects: 16S rDNA; Animals; Arcanobacterium - cytology; Arcanobacterium - genetics; Arcanobacterium pyogenes; Bacterial Proteins - genetics; Bacterial Toxins - genetics; bearded dragon; Cluster Analysis; Diagnosis; DNA Primers - genetics; DNA, Ribosomal - genetics; gecko; Genotype; Hemolysin Proteins - genetics; IDENTIFICACION; IDENTIFICATION; Lizards - microbiology; Molecular Sequence Data; Phenotype; rpoB; Sequence Analysis, DNA; Short Communication; Species Specificity; 수의학
• ISSN: 1229-845X; 1976-555X
• DOI: 10.4142/jvs.2010.11.3.265

The present study was designed to identify phenotypically and genotypically two Arcanobacterium (A.) pyogenes strains isolated by post mortem examinations of a bearded dragon and a gecko. The A. pyogenes strains showed the typical biochemical properties and displayed CAMP-like synergistic hemolytic activities with various indicator strains. The species identity could be confirmed genotypically by amplification and sequencing of the 16S rDNA gene and, as novel target gene, by sequencing or the beta subunit of RNA polymerase encoding gene rpoB, of both strains and of reference strains representing nine species of the genus Arcanobacterium. The species identity or the two A. pyogenes strains could additionally be confirmed by PCR mediated amplification of species specific parts of the 16S-23S rDNA intergenic spacer region, the pyolysin encoding gene plo and by amplification of the collagen-binding protein encoding gene cbpA. All these molecular targets might help to improve the future identification and further characterization of A. pyogenes which, as demonstrated in the present study, could also be isolated from reptile specimens.