Ethanol extract of Angelica gigas inhibits croton oil-induced inflammation by suppressing the cyclooxygenase - prostaglandin pathway

• Published in: Journal of veterinary science (Suwŏn-si, Korea) 2010, 11(1), , pp.43-50
• Main Authors: Shin, S.H., Chungbuk National University, Cheongju, Republic of Korea, Joo, S.S., Chungbuk National University, Cheongju, Republic of Korea, Park, D.S., Chungbuk National University, Cheongju, Republic of Korea, Jeon, J.H., Chungbuk National University, Cheongju, Republic of Korea, Kim, T.K., Chungbuk National University, Cheongju, Republic of Korea, Kim, J.S., Daejeon Health Sciences College, Daejeon, Republic of Korea, Park, S.K., Daejeon Health Sciences College, Daejeon, Republic of Korea, Hwang, B.Y., Chungbuk National University, Cheongju, Republic of Korea, Kim, Y.B., Chungbuk National University, Cheongju, Republic of Korea
• Format: Article
• Language: English
• Subjects: Angelica - immunology; Angelica gigas; Animals; Cell Line; croton oil; Cyclooxygenase 1 - genetics; Cyclooxygenase 1 - immunology; Cyclooxygenase 2 - genetics; Cyclooxygenase 2 - immunology; Dinoprostone - genetics; Dinoprostone - immunology; INFLAMACION; INFLAMMATION; Inflammation - drug therapy; Inflammation - enzymology; Inflammation - immunology; Interleukin-6 - blood; Macrophages; Male; Mice; Mice, Inbred ICR; Nitric Oxide - blood; Original; Phytotherapy - methods; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Plant Roots - immunology; prostaglandin E-2; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - chemistry; RNA, Messenger - genetics; Tumor Necrosis Factor-alpha - blood; 수의학
• ISSN: 1229-845X; 1976-555X
• DOI: 10.4142/jvs.2010.11.1.43

The anti-inflammatory effects of an ethanol extract of Angelica gigas (EAG), were investigated in vitro and in vivo, using croton oil-induced inflammation models. Croton oil (20 ㎍/mL) up-regulated mRNA expression of cyclooxygenase (COX)-Ⅰ and COX-Ⅱ in the macrophage cell line, RAW 264.7, resulting in the release of high concentrations or prostaglandin E₂ (PGE₂). EAG (1~10 ㎍/mL) markedly suppressed croton oil-induced COX-Ⅱ mRNA expression and PGE₂ production. Application of croton oil (5% in acetone) to mouse ears caused severe local erythema, edema and vascular leakage, which were significantly attenuated by oral pre-treatment with EAG (50~500 mg/kg). Croton oil dramatically increased blood levels of interleukin (IL)-6 and PGE₂ without affecting tumor-necrosis factor (TNF)-α and nitric oxide (NO) levels. EAG pre-treatment remarkably lowered IL-6 and PGE₂, but did not alter TNF-α or NO concentrations. These results indicate that EAG attenuates inflammatory responses in part by blocking the COX - PGE₂ pathway. Therefore, EAG could be a promising candidate for the treatment or inflammatory diseases.