Chloroform induces cystein-rich 61, a mediator of collagen homeostasis via early growth response-1 dependent pathway in human skin dermal fibroblasts

Volatile organic compounds (VOCs) are major air pollutants known to have adverse effects on skin. Many reports have shown an association between air pollutants and skin aging. However, there is no report on VOCs association with skin aging. The aim of this study is to identify the biologic effects o...

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Bibliographic Details
Published in:Molecular & cellular toxicology 2016, 12(4), , pp.337-343
Main Authors: Lee, Hana, Park, Jae Beom, Ryu, Woo-In, Kim, Ji Hyun, Shin, Jung Jin, Son, Sang Wook
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Cell Biology
Chloroform
Collagen
CYR61 protein
Cysteine
EGR-1 protein
Fibroblasts
Homeostasis
Interstitial collagenase
Life Sciences
Matrix metalloproteinase
Metalloproteinase
Organic compounds
Original Paper
Pharmacology/Toxicology
Pollutants
Procollagen
Side effects
siRNA
Skin
VOCs
Volatile organic compounds
생물학
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Summary:Volatile organic compounds (VOCs) are major air pollutants known to have adverse effects on skin. Many reports have shown an association between air pollutants and skin aging. However, there is no report on VOCs association with skin aging. The aim of this study is to identify the biologic effects of VOCs on fibroblasts. We investigated the effects of chloroform on cysteine-rich 61 (CYR61) expression in human skin fibroblasts using western blot and reverse transcriptionpolymerase chain reaction. We also observed the change of type I procollagen and matrix metalloproteinase after exposure to chloroform using western blot. We explored the involvement of early growth response-1 (Egr-1) using siRNA s in the fibroblasts. Chloroform exposure increased the expression of CYR61 protein and mRNA level. Elevated CYR61 downregulates type I procollagen and upregulates MMP-1. Egr-1 overexpression upregulates CYR61 promoter activity. CYR61 promoter activity was lower in Egr-1 siRNA-transfected fibroblasts. The present study demonstrates that CYR61 is transcriptionally regulated by chloroform exposure through transcription factor Egr-1, and alters collagen homeostasis in human skin fibroblasts.
ISSN:1738-642X
2092-8467
DOI:10.1007/s13273-016-0038-6