HSP60 is required for stemness and proper differentiation of mouse embryonic stem cells

Embryonic stem cells (ESCs) are metabolically distinct from their differentiated counterparts. ESC mitochondria are less complex and fewer in number than their differentiated progeny. However, few studies have examined the proteins responsible for differences in mitochondrial structure and function...

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Bibliographic Details
Published in:Experimental & molecular medicine 2018, 50(0), , pp.1-8
Main Authors: 서난희, 이은혜, 서진희, 송은경, 한명관
Format: Article
Language:English
Subjects:
생화학
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Summary:Embryonic stem cells (ESCs) are metabolically distinct from their differentiated counterparts. ESC mitochondria are less complex and fewer in number than their differentiated progeny. However, few studies have examined the proteins responsible for differences in mitochondrial structure and function between ESCs and somatic cells. Therefore, in this study, we aimed to investigate the differences between mitochondrial proteins in these two cell types. We demonstrate that HSP60 is more abundant in mouse ESC mitochondria than in mouse embryonic fibroblasts. Depletion of HSP60 inhibited mouse ESC proliferation and self-renewal, characterized by decreased OCT4 expression. HSP60 depletion also enhanced apoptosis during mouse ESC differentiation into embryoid bodies. Our results suggest that HSP60 expression has an essential role in ESC selfrenewal and survival of differentiated cells from ESCs. KCI Citation Count: 0
ISSN:1226-3613
2092-6413