Re-evaluation of physicochemical and NMR data of triol ginsenosides Re, Rf, Rg2, and 20-gluco-Rf from Panax ginseng roots

Ginseng roots were extracted with aqueous methanol, and extracts were suspended in water and extracted successively with ethyl acetate and n-butanol. Column chromatography using the n-butanol fraction yielded four purified triol ginseng saponins: the ginsenosides Re, Rf, Rg2, and 20-gluco-Rf. The ph...

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Bibliographic Details
Published in:Journal of ginseng research 2014, 38(2), , pp.116-122
Main Authors: Cho, Jin-Gyeong, In, Seo-Ji, Jung, Ye-Jin, Cha, Byeong-Ju, Lee, Dae-Young, Kim, Yong-Bum, Yeom, Myeonghun, Baek, Nam-In
Format: Article
Language:English
Subjects:
fast atom bombardment/MS
HPLC
NMR
Panax ginseng
triol ginsenoside
기타의약학
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Summary:Ginseng roots were extracted with aqueous methanol, and extracts were suspended in water and extracted successively with ethyl acetate and n-butanol. Column chromatography using the n-butanol fraction yielded four purified triol ginseng saponins: the ginsenosides Re, Rf, Rg2, and 20-gluco-Rf. The physicochemical, spectroscopic, and chromatographic characteristics of the ginsenosides were measured and compared with reports from the literature. For spectroscopic analysis, two-dimensional nuclear magnetic resonance (NMR) methods such as 1H-1H correlation spectroscopy, nuclear Overhauser effect spectroscopy, heteronuclear single quantum correlation, and heteronuclear multiple bond connectivity were employed to identify exact peak assignments. Some peak assignments for previously published 1H- and 13C-NMR spectra were found to be inaccurate. This study reports the complete NMR assignment of 20-gluco-Rf for the first time.
ISSN:1226-8453
2093-4947
DOI:10.1016/j.jgr.2013.10.002