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Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs

Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental dat...

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Published in:Journal of veterinary science (Suwŏn-si, Korea) 2018, 19(3), , pp.350-357
Main Authors: Rahpaya, Sayed Samim, Tsuchiaka, Shinobu, Kishimoto, Mai, Oba, Mami, Katayama, Yukie, Nunomura, Yuka, Kokawa, Saki, Kimura, Takashi, Kobayashi, Atsushi, Kirino, Yumi, Okabayashi, Tamaki, Nonaka, Nariaki, Mekata, Hirohisa, Aoki, Hiroshi, Shiokawa, Mai, Umetsu, Moeko, Morita, Tatsushi, Hasebe, Ayako, Otsu, Keiko, Asai, Tetsuo, Yamaguchi, Tomohiro, Makino, Shinji, Murata, Yoshiteru, Abi, Ahmad Jan, Omatsu, Tsutomu, Mizutani, Tetsuya
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cited_by cdi_FETCH-LOGICAL-c529t-a863ce22b34fec41b9f0bdcadb5ad5d09327f7fb568912afd16745e21b74d93b3
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container_title Journal of veterinary science (Suwŏn-si, Korea)
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creator Rahpaya, Sayed Samim
Tsuchiaka, Shinobu
Kishimoto, Mai
Oba, Mami
Katayama, Yukie
Nunomura, Yuka
Kokawa, Saki
Kimura, Takashi
Kobayashi, Atsushi
Kirino, Yumi
Okabayashi, Tamaki
Nonaka, Nariaki
Mekata, Hirohisa
Aoki, Hiroshi
Shiokawa, Mai
Umetsu, Moeko
Morita, Tatsushi
Hasebe, Ayako
Otsu, Keiko
Asai, Tetsuo
Yamaguchi, Tomohiro
Makino, Shinji
Murata, Yoshiteru
Abi, Ahmad Jan
Omatsu, Tsutomu
Mizutani, Tetsuya
description Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental data supporting this possibility are scarce. We collected 117 samples and screened them for 44 bovine abortive, diarrheal, and respiratory disease complex pathogens by using Dembo polymerase chain reaction (PCR), which is based on TaqMan real-time PCR. Fifty-seven samples were positive for at least one pathogen, including bovine viral diarrhea virus, bovine enterovirus, ser. Dublin, ser. Typhimurium, and ; some samples were positive for multiple pathogens. Bovine viral diarrhea virus and bovine enterovirus were the most frequently detected pathogens, especially in flies, suggesting an important role of flies in the transmission of these viruses. Additionally, we detected the genome from a cockroach sample for the first time. Our data suggest that insects (particularly flies), birds, and rodents are potential vectors and reservoirs of abortion, diarrhea, and respiratory infectious agents, and that they may transmit more than one pathogen at the same time.
doi_str_mv 10.4142/jvs.2018.19.3.350
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subjects Abortion, Veterinary - diagnosis
Animals
Birds - microbiology
Birds - virology
Cattle
Cattle Diseases - diagnosis
Diarrhea - diagnosis
Diarrhea - veterinary
Disease Reservoirs
Disease Vectors
Insecta - microbiology
Insecta - virology
Original
Real-Time Polymerase Chain Reaction - methods
Real-Time Polymerase Chain Reaction - veterinary
Respiratory Tract Diseases - diagnosis
Respiratory Tract Diseases - veterinary
Rodentia - microbiology
Rodentia - virology
수의학
title Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs
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