Roseovarius tibetensis sp. nov., a halophilic bacterium isolated from Lake LongmuCo on Tibetan Plateau

• Published in: The journal of microbiology 2018, 56(11), , pp.783-789
• Main Authors: Lu, Hui-bin, Xue, Xiao-feng, Phurbu, Dorji, Xing, Peng, Wu, Qing-long
• Format: Article
• Language: English
• Subjects: Bacterial Typing Techniques; Base Composition; Biochemical characteristics; Biochemistry; Biomedical and Life Sciences; Cardiolipin; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Fatty acids; Fatty Acids - analysis; Gene sequencing; Genes, Bacterial - genetics; Glucosamine; Hybridization; Lakes; Lakes - chemistry; Lakes - microbiology; Lecithin; Life Sciences; Lipids; Microbial Systematics and Evolutionary Microbiology; Microbiology; New species; Nucleic Acid Hybridization; Nucleotide sequence; Phosphatidylcholine; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids; Phospholipids - analysis; Phylogenetics; Phylogeny; Quinones; Quinones - analysis; Rhodobacteraceae - classification; Rhodobacteraceae - genetics; Rhodobacteraceae - isolation & purification; Rhodobacteraceae - physiology; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Sequencing; Similarity; Species classification; Species Specificity; Strains (organisms); Tibet; Ubiquinone; Water Microbiology; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-018-8178-0

Two Gram-stain negative halophilic strains, designated as LM2 T and LM4, were isolated from Lake LongmuCo on Tibetan Plateau. These two strains were aerobic, catalaseand oxidase-positive, nonmotile and rod-shaped organisms. Phylogenetic analysis based on 16S rRNA gene sequences indicated that LM2 T and LM4 belong to the genus Roseovarius , with Roseovarius tolerans EL-172 T (97.3% and 97.4% 16S rRNA gene sequence similarity, respectively) and Roseovarius azorensis SSW084 T (95.5% and 95.6% 16S rRNA gene sequence similarity, respectively) as their closest neighbors. Q-10 was the sole respiratory quinone of these two strains. The major fatty acids were C 18:1 ω 7 c /C 18:1 ω 6 c , C 16:0 , C 19:0 cyclo ω 8 c , and 11-methyl C 18:1 ω 7 c . The polar lipids included phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phospholipid of unknown structure containing glucosamine, and unidentified aminolipid. The DNA G + C content was between 64.2 and 64.5 mol%. DNA-DNA hybridization showed 96.7% relatedness between LM2 T and LM4, 24.9% relatedness between LM2 T and R. tolerans EL-172 T , and 36.3% relatedness between LM4 and R. tolerans EL-172 T . Based on phylogenetic analysis, DNA-DNA hybridization, a range of physiological and biochemical characteristics, LM2 T and LM4 belong to the same species and were clearly distinguished from the type strains of the genus Roseovarius . It was evident that LM2 T and LM4 could be classified as a novel species of the genus Roseovarius, for which the name Roseovarius tibetensis sp. nov. is proposed. The type strain is LM2 T (= CGMCC 1.16230 T = KCTC 62028 T ).