Kinetic analysis of 64Cu-NODAGA-gluco-E[c(RGDfK)]2 for a tumor angiogenesis PET tracer

• Published in: 대한방사성의약품학회지, 2(2) 2016, 2(2), , pp.108-112
• Main Authors: 최재용, 박지애, 김정영, 이지웅, 이민경, 신운철, 강주현, 안광일, 이교철, 류영훈, 김경민
• Format: Article
• Language: Korean
• Subjects: 방사선과학
• ISSN: 2384-1583; 2508-3848

Molecular imaging with the radiolabeled RGD peptides for αvβ3 integrin has been an increasing interest for tumor diagnosis and the treatment monitoring. Recently, 64Cu-NODAGA-gluco-E[c(RGDfK)]2 was developed for quantification of αvβ3 integrin and its biological properties was elucidated. To better understand the molecular process in vivo, we performed the kinetic analysis for the 64Cu-NODAGA-gluco-E[c(RGDfK)]2. After preparation of a radiotracer, dynamic PET images were obtained in the U87MG xenograft mice for 60 min (n = 6). Binding potential values were estimated from the 3-tissue compartment model, reference Logan and simplified reference tissue model. In the early time frame (0-20 min), the liver, kidney, intestine, urinary bladder and tumor were visualized but these uptakes were diminished as time went by. The tumors showed a good contrast at 40 min after administration. 64Cu-NODAGA-E[c(RGDfK)]2 showed the 2-fold uptake in the tumor compared with that in the muscle. The parametric maps for binding values also provide the higher tumor-tobackground contrast than the static images. A binding value obtained from the 3-tissue compartment model was comparable to other modeling methods. From these results, we conclude that 64Cu-NODAGA-glucoE[c(RGDfK)]2 may be a promising PET radiotracer for the evaluation of angiogenesis. J Radiopharm Mol Probes 2(2):108-112, 2016 KCI Citation Count: 0