Synthesis of (R,R)-2,3-butanediol from starch in a hybrid cell-free reaction system

In this study, we demonstrate the conversion of starch to (R,R)-2,3-butanediol (2,3-BD) in a hybrid cell-free synthesis system containing a mixture of lysates derived from Escherichia coli (E. coli) and cyanobacteria. A sufficient pool of pyruvate required for the synthesis of 2,3-BD was generated b...

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Bibliographic Details
Published in:Journal of industrial and engineering chemistry (Seoul, Korea) 2018, 67(0), , pp.231-235
Main Authors: Yi, Tong, Lim, Hye Jin, Lee, So Jeong, Lee, Kyung-Ho, Kim, Dong-Myung
Format: Article
Language:English
Subjects:
화학공학
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Summary:In this study, we demonstrate the conversion of starch to (R,R)-2,3-butanediol (2,3-BD) in a hybrid cell-free synthesis system containing a mixture of lysates derived from Escherichia coli (E. coli) and cyanobacteria. A sufficient pool of pyruvate required for the synthesis of 2,3-BD was generated by combining metabolic pathways of cyanobacteria and E. coli. Successful synthesis of 2,3-BD was achieved by additional modifications of the hybrid cell-free system with the enzymes required to convert pyruvate to 2,3-BD. The results demonstrate a new approach to harness biological pathways to expand the scope of cell-free metabolic engineering by cross-species combinations of cell lysates. KCI Citation Count: 7
ISSN:1226-086X
1876-794X
DOI:10.1016/j.jiec.2018.06.033