Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification

(ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombin...

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Bibliographic Details
Published in:The plant pathology journal 2018, 34(6), , pp.575-579
Main Authors: Kim, Nam-Yeon, Oh, Jonghee, Lee, Su-Heon, Kim, Hongsup, Moon, Jae Sun, Jeong, Rae-Dong
Format: Article
Language:English
Subjects:
Apple stem grooving virus
molecular diagnosis
reverse transcription-recombinase polymerase amplification
농학
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Summary:(ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA obtained from an ASGV-infected plant. The major advantage of this assay is that the reaction for the target virus is completed in 1 min, and amplification only requires an incubation temperature of 42°C. This assay is a promising alternative method for pear breeding programs or virus-free certification laboratories.
ISSN:1598-2254
2093-9280
DOI:10.5423/PPJ.NT.06.2018.0108