Evaluation of in vitro anti-oxidant and anti-inflammatory activities of Korean and Chinese Lonicera caerulea

• Published in: Nutrition research and practice 2018, 12(6), , pp.486-493
• Main Authors: Lee, You-Suk, Cho, Il Je, Kim, Joo Wan, Lee, Sun-Kyoung, Ku, Sae Kwang, Lee, Hae-Jeung
• Format: Article
• Language: English
• Subjects: Original Research; 생활과학
• ISSN: 1976-1457; 2005-6168
• DOI: 10.4162/nrp.2018.12.6.486

The honeysuckle berry (HB) contains ascorbic acid and phenolic components, especially anthocyanins, flavonoids, and low-molecular-weight phenolic acids. In order to examine the potential of HB as a hepatoprotective medicinal food, we evaluated the anti-oxidant and anti-inflammatory activities of Korean HB (HBK) and Chinese HB (HBC). Antioxidant and anti-inflammatory effects of the extracts were examined in HepG2 and RAW 264.7 cells, respectively. The anti-oxidant capacity was determined by DPPH, SOD, CAT, and ARE luciferase activities. The production of nitric oxide (NO) as an inflammatory marker was also evaluated. The -mediated mRNA levels of heme oxygenase-1 ( ), NAD(P)H dehydrogenase [quinone] 1 ( ), and glutamate-cysteine ligase catalytic subunit ( ) were measured. The concentrations of HB extracts used were 3, 10, 30, 100, and 300 µg/mL. The radical scavenging activity of all HB extracts increased in a concentration-dependent manner ( < 0.01 or < 0.05). SOD ( < 0.05) and CAT ( < 0.01) activities were increased by treatment with 300 µg/mL of each HB extract, when compared to those in the control. NO production was observed in cells pretreated with 100 or 300 µg/mL of HBC and HBK ( < 0.01). Treatment with 300 µg/mL of HBC significantly increased ( < 0.01) and ( < 0.05) mRNA levels compared to those in the control. Treatment with 300 µg/mL of HBK ( < 0.05) and HBC ( < 0.01) also significantly increased the mRNA level compared to that in the control. Thus, the Korean and Chinese HBs were found to possess favorable anti-oxidant and anti-inflammatory activities. and its related anti-oxidant genes were associated with both anti-oxidant and anti-inflammatory activities in HB-treated cells. Further studies are needed to confirm these effects.