Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm

• Published in: Journal of veterinary science (Suwŏn-si, Korea) 2019, 20(1), , pp.79-86
• Main Authors: Abdillah, Dimas A, Setyawan, Erif M N, Oh, Hyun Ju, Ra, Kihae, Lee, Seok Hee, Kim, Min Jung, Lee, Byeong Chun
• Format: Article
• Language: English
• Subjects: Animals; Cryopreservation - veterinary; DNA Damage - drug effects; Dogs; Dose-Response Relationship, Drug; Male; Original; Protamines - metabolism; Protective Agents - pharmacology; Reactive Oxygen Species - metabolism; Semen Preservation - veterinary; Spermatozoa - drug effects; Spermatozoa - physiology; Triiodobenzoic Acids - pharmacology; 수의학
• ISSN: 1229-845X; 1976-555X
• DOI: 10.4142/jvs.2019.20.1.79

The objective of this study was to analyze the protective effects of iodixanol on dog spermatozoa during cryopreservation. The optimal concentration of iodixanol, 1.5%, was determined using fresh spermatozoa and was applied in the following experiments. The 1.5% iodixanol group showed significantly increased sperm motility from that in the control ( ＜ 0.05). Lower mitochondrial reactive oxygen species (ROS) modulator ( ) and pro-apoptotic gene ( ) expressions, together with higher expressions of protamine-2 ( ), protamine-3 ( ), anti-apoptotic B-cell lymphoma-2 ( ), and sperm acrosome associated-3 ( ) genes were detected in the iodixanol-treated group. In addition, decreased protamine deficiency and cryocapacitation were observed in the treatment group. Our results show that supplementation with 1.5% iodixanol is ideal for reducing production of ROS and preventing detrimental effects during the canine sperm cryopreservation process, effects manifested as increased motility and reduced cryocapacitation in frozen-thawed spermatozoa.