Paraburkholderia dokdonella sp. nov., isolated from a plant from the genus Campanula

• Published in: The journal of microbiology 2019, 57(2), , pp.107-112
• Main Authors: Jung, Man-Young, Kang, Myung-Suk, Lee, Ki-Eun, Lee, Eun-Young, Park, Soo-Je
• Format: Article
• Language: English
• Subjects: Aldehydes; Biomedical and Life Sciences; Campanula; Comparative analysis; Deoxyribonucleic acid; DNA; E coli; Fatty acids; Gene amplification; Gram-negative bacteria; Indigenous plants; Life Sciences; Lipids; Microbial Systematics and Evolutionary Microbiology; Microbiological strains; Microbiology; Morphology; New species; Nucleotide sequence; Paraburkholderia; pH effects; Phosphatidylethanolamine; Phosphatidylglycerol; Phylogenetics; Physiology; Quinones; rRNA 16S; Sequencing; Sodium chloride; Temperature; Ubiquinone; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-019-8500-5

The novel Gram-stain-negative, rod-shaped, aerobic bacterial strain DCR-13 T was isolated from a native plant belonging to the genus Campanula on Dokdo, an island in the Republic of Korea. Comparative analysis of the 16S rRNA gene sequence indicated that this strain is closely related to Paraburkholderia peleae PP52-1 T (98.43% 16S rRNA gene sequence similarity), Paraburkholderia oxyphila NBRC 105797 T (98.42%), Paraburkholderia sacchari IPT 101 T (98.28%), Paraburkholderia mimosarum NBRC 106338 T (97.80%), Paraburkholderia denitrificans KIS30-44 T (97.46%), and Paraburkholderia paradise WA T (97.45%). This analysis of the 16S rRNA gene sequence also suggested that DCR-13 T and the six closely related strains formed a clade within the genus Paraburkholderia , but that DCR-13 T was clearly separated from the established species. DCR-13 T had ubiquinone 8 as its predominant respiratory quinone, and its genomic DNA G + C content was 63.9 mol%. The isolated strain grew at a pH of 6.0–8.0 (with an optimal pH of 6.5), 0–4% w/v NaCl (with an optimal level of 0%), and a temperature of 18–42°C (with an optimal temperature of 30°C). The predominant fatty acids were C 16:0 , summed feature 8 (C 18:1 ω 7 c /C 18:1 ω 6 c ), C 17:0 cyclo, C 19:0 cyclo ω 8 c , summed feature 3 (C 16:1 ω 6 c /C 16:1 ω 7 c ) and summed feature 2 (C 12:0 aldehyde), and the major polar lipids were phosphatidylglycerol and phosphatidylethanolamine. On the basis of polyphasic evidence, it is proposed that strain DCR-13 T (= KCTC 62811 T = LMG 30889 T ) represents the type strain of a novel species, Paraburkholderia dokdonella sp. nov.