Digital Holographic Microscopy with extended field of view using tool for generic image stitching

This paper describes in detail the processing path leading to successful phase images stitching in digital holographic microscope for the extension of the field of view. It applies FIJI Grid/Collection Stitching Plugin, which is a general tool for images stitching, non‐specific for phase images. The...

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Bibliographic Details
Published in:ETRI journal 2019, 41(1), , pp.73-83
Main Authors: Stępień, Piotr, Korbuszewski, Damian, Kujawińska, Małgorzata
Format: Article
Language:English
Subjects:
aberration correction
cell culture analysis
digital holographic microscopy
digital holography
stitching
전자/정보통신공학
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Summary:This paper describes in detail the processing path leading to successful phase images stitching in digital holographic microscope for the extension of the field of view. It applies FIJI Grid/Collection Stitching Plugin, which is a general tool for images stitching, non‐specific for phase images. The FIJI plugin is extensively supported by aberration and phase offset correction. Comparative analysis of different aberration correction methods and data processing strategies is presented, together with the critical analysis of their applicability. The proposed processing path provides good background for statistical phase analysis of cell cultures and digital phase pathology.
ISSN:1225-6463
2233-7326
DOI:10.4218/etrij.2018-0499