A LIM Protein Gene Homologue of Protaetia brevitarsis cDNA Cloning and mRNA Expression

A LIM protein gene homologue of the CRP (cysteine-rich protein) family in the whiter-spotted flower chafer, Protaetia brevitarsis, was cloned. The P. brevitarsis LIM protein cDNA encodes a 92 amino acid polypeptide with a predicted molecular mass of 10,030 Da and a pI of 8.57. The P. brevitarsis LIM...

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Bibliographic Details
Published in:International Journal of Industrial Entomology 2005, 11(1), , pp.71-74
Main Authors: Ik Soo Kim, Yong Soo Choi, Sun Young Lee, Mi Ae Kim, Seong Ryul Kim, Jae Sam Hwang, Byung Rae Jin
Format: Article
Language:Korean
Subjects:
cDNA cloning
Insect
LIM protein
Prota­etia brevitarsis
농학
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Summary:A LIM protein gene homologue of the CRP (cysteine-rich protein) family in the whiter-spotted flower chafer, Protaetia brevitarsis, was cloned. The P. brevitarsis LIM protein cDNA encodes a 92 amino acid polypeptide with a predicted molecular mass of 10,030 Da and a pI of 8.57. The P. brevitarsis LIM protein contains the cysteine-rich consensus sequence of LIM domain and the glycine-rich consensus sequence observed in the cysteine-rich protein family 1 (CRP1). The potential nuclear targeting signal is retained. The deduced amino acid sequence of the P. brevitarsis LIM protein cDNA showed 92% identity to another beetle, Apriona germari LIM protein. Northern blot analysis showed that P. brevitarsis LIM protein is highly expressed in epidermis and midgut, but not in the fat body.
ISSN:1598-3579
2586-4785