Loading…

Specification of Bacteriophage Isolated Against Clinical Methicillin-Resistant Staphylococcus Aureus

The emergence of resistant bacteria is being increasingly reported around the world, potentially threatening millions of lives. Amongst resistant bacteria, methicillin-resistant (MRSA) is the most challenging to treat. This is due to emergent MRSA strains and less effective traditional antibiotic th...

Full description

Saved in:
Bibliographic Details
Published in:Osong public health and research perspectives 2019, 10(1), , pp.20-24
Main Authors: Nasser, Ahmad, Azizian, Reza, Tabasi, Mohsen, Khezerloo, Jamil Kheirvari, Heravi, Fatemah Sadeghpour, Kalani, Morovat Taheri, Sadeghifard, Norkhoda, Amini, Razieh, Pakzad, Iraj, Radmanesh, Amin, Jalilian, Farid Azizi
Format: Article
Language:English
Subjects:
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c3575-584130c2d843982ee21d15ea9fd84161a5b8f45e709acce3654d39ecd11113893
cites
container_end_page 24
container_issue 1
container_start_page 20
container_title Osong public health and research perspectives
container_volume 10
creator Nasser, Ahmad
Azizian, Reza
Tabasi, Mohsen
Khezerloo, Jamil Kheirvari
Heravi, Fatemah Sadeghpour
Kalani, Morovat Taheri
Sadeghifard, Norkhoda
Amini, Razieh
Pakzad, Iraj
Radmanesh, Amin
Jalilian, Farid Azizi
description The emergence of resistant bacteria is being increasingly reported around the world, potentially threatening millions of lives. Amongst resistant bacteria, methicillin-resistant (MRSA) is the most challenging to treat. This is due to emergent MRSA strains and less effective traditional antibiotic therapies to Staphylococcal infections. The use of bacteriophages (phages) against MRSA is a new, potential alternate therapy. In this study, morphology, genetic and protein structure of lytic phages against MRSA have been analysed. Isolation of livestock and sewage bacteriophages were performed using 0.4 μm membrane filters. Plaque assays were used to determine phage quantification by double layer agar method. Pure plaques were then amplified for further characterization. Sulfate-polyacrylamide gel electrophoresis and random amplification of polymorphic DNA were run for protein evaluation, and genotyping respectively. Transmission electron microscope was also used to detect the structure and taxonomic classification of phage visually. Head and tail morphology of bacteriophages against MRSA were identified by transmission electron microscopy and assigned to the family and the order. Bacteriophages are the most abundant microorganism on Earth and coexist with the bacterial population. They can destroy bacterial cells successfully and effectively. They cannot enter mammalian cells which saves the eukaryotic cells from lytic phage activity. In conclusion, phage therapy may have many potential applications in microbiology and human medicine with no side effect on eukaryotic cells.
doi_str_mv 10.24171/j.phrp.2019.10.1.05
format article
fullrecord <record><control><sourceid>proquest_nrf_k</sourceid><recordid>TN_cdi_nrf_kci_oai_kci_go_kr_ARTI_4402302</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2189533923</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3575-584130c2d843982ee21d15ea9fd84161a5b8f45e709acce3654d39ecd11113893</originalsourceid><addsrcrecordid>eNpVUV1r3DAQFKWlCdf8g1L82D7Y1adtvRSuR9scpBSS9Fko8vqsi85yJTmQf1_5LgntItjV7MxIMAi9J7iinDTk876ahjBVFBNZZZBUWLxC55QyVtZY0NfLTHApsZRn6CLGPc6VN7UUb9EZwy1vaN2co-5mAmN7a3Syfix8X3zVJkGwfhr0Dopt9E4n6Ir1TtsxpmLj7JjZrvgJabDGunwvryHamPSYipukp-HReeONmWOxngPM8R1602sX4eKpr9Dv799uN5fl1a8f2836qjRMNKIULScMG9q1nMmWAlDSEQFa9hkhNdHiru25gAZLbQywWvCOSTAdycVayVbo08l3DL26N1Z5bY9959V9UOvr263iHFOWzwp9OXGn-e4AnYExBe3UFOxBh8ej8v_NaIfs86BqJuuWLgYfnwyC_zNDTOpgowHn9Ah-joqSVgrGZI5khfiJaoKPMUD_8gzB6pin2qslT7XkuYBEYZFlH_794ovoOT32F7u-noU</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2189533923</pqid></control><display><type>article</type><title>Specification of Bacteriophage Isolated Against Clinical Methicillin-Resistant Staphylococcus Aureus</title><source>PubMed Central (Training)</source><creator>Nasser, Ahmad ; Azizian, Reza ; Tabasi, Mohsen ; Khezerloo, Jamil Kheirvari ; Heravi, Fatemah Sadeghpour ; Kalani, Morovat Taheri ; Sadeghifard, Norkhoda ; Amini, Razieh ; Pakzad, Iraj ; Radmanesh, Amin ; Jalilian, Farid Azizi</creator><creatorcontrib>Nasser, Ahmad ; Azizian, Reza ; Tabasi, Mohsen ; Khezerloo, Jamil Kheirvari ; Heravi, Fatemah Sadeghpour ; Kalani, Morovat Taheri ; Sadeghifard, Norkhoda ; Amini, Razieh ; Pakzad, Iraj ; Radmanesh, Amin ; Jalilian, Farid Azizi</creatorcontrib><description>The emergence of resistant bacteria is being increasingly reported around the world, potentially threatening millions of lives. Amongst resistant bacteria, methicillin-resistant (MRSA) is the most challenging to treat. This is due to emergent MRSA strains and less effective traditional antibiotic therapies to Staphylococcal infections. The use of bacteriophages (phages) against MRSA is a new, potential alternate therapy. In this study, morphology, genetic and protein structure of lytic phages against MRSA have been analysed. Isolation of livestock and sewage bacteriophages were performed using 0.4 μm membrane filters. Plaque assays were used to determine phage quantification by double layer agar method. Pure plaques were then amplified for further characterization. Sulfate-polyacrylamide gel electrophoresis and random amplification of polymorphic DNA were run for protein evaluation, and genotyping respectively. Transmission electron microscope was also used to detect the structure and taxonomic classification of phage visually. Head and tail morphology of bacteriophages against MRSA were identified by transmission electron microscopy and assigned to the family and the order. Bacteriophages are the most abundant microorganism on Earth and coexist with the bacterial population. They can destroy bacterial cells successfully and effectively. They cannot enter mammalian cells which saves the eukaryotic cells from lytic phage activity. In conclusion, phage therapy may have many potential applications in microbiology and human medicine with no side effect on eukaryotic cells.</description><identifier>ISSN: 2210-9099</identifier><identifier>EISSN: 2233-6052</identifier><identifier>DOI: 10.24171/j.phrp.2019.10.1.05</identifier><identifier>PMID: 30847267</identifier><language>eng</language><publisher>Korea (South): Korea Centers for Disease Control and Prevention</publisher><subject>Original ; 예방의학</subject><ispartof>Osong Public Health and Research Perspectives, 2019, 10(1), , pp.20-24</ispartof><rights>Copyright ©2019, Korea Centers for Disease Control and Prevention 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3575-584130c2d843982ee21d15ea9fd84161a5b8f45e709acce3654d39ecd11113893</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396822/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396822/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30847267$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART002438467$$DAccess content in National Research Foundation of Korea (NRF)$$Hfree_for_read</backlink></links><search><creatorcontrib>Nasser, Ahmad</creatorcontrib><creatorcontrib>Azizian, Reza</creatorcontrib><creatorcontrib>Tabasi, Mohsen</creatorcontrib><creatorcontrib>Khezerloo, Jamil Kheirvari</creatorcontrib><creatorcontrib>Heravi, Fatemah Sadeghpour</creatorcontrib><creatorcontrib>Kalani, Morovat Taheri</creatorcontrib><creatorcontrib>Sadeghifard, Norkhoda</creatorcontrib><creatorcontrib>Amini, Razieh</creatorcontrib><creatorcontrib>Pakzad, Iraj</creatorcontrib><creatorcontrib>Radmanesh, Amin</creatorcontrib><creatorcontrib>Jalilian, Farid Azizi</creatorcontrib><title>Specification of Bacteriophage Isolated Against Clinical Methicillin-Resistant Staphylococcus Aureus</title><title>Osong public health and research perspectives</title><addtitle>Osong Public Health Res Perspect</addtitle><description>The emergence of resistant bacteria is being increasingly reported around the world, potentially threatening millions of lives. Amongst resistant bacteria, methicillin-resistant (MRSA) is the most challenging to treat. This is due to emergent MRSA strains and less effective traditional antibiotic therapies to Staphylococcal infections. The use of bacteriophages (phages) against MRSA is a new, potential alternate therapy. In this study, morphology, genetic and protein structure of lytic phages against MRSA have been analysed. Isolation of livestock and sewage bacteriophages were performed using 0.4 μm membrane filters. Plaque assays were used to determine phage quantification by double layer agar method. Pure plaques were then amplified for further characterization. Sulfate-polyacrylamide gel electrophoresis and random amplification of polymorphic DNA were run for protein evaluation, and genotyping respectively. Transmission electron microscope was also used to detect the structure and taxonomic classification of phage visually. Head and tail morphology of bacteriophages against MRSA were identified by transmission electron microscopy and assigned to the family and the order. Bacteriophages are the most abundant microorganism on Earth and coexist with the bacterial population. They can destroy bacterial cells successfully and effectively. They cannot enter mammalian cells which saves the eukaryotic cells from lytic phage activity. In conclusion, phage therapy may have many potential applications in microbiology and human medicine with no side effect on eukaryotic cells.</description><subject>Original</subject><subject>예방의학</subject><issn>2210-9099</issn><issn>2233-6052</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNpVUV1r3DAQFKWlCdf8g1L82D7Y1adtvRSuR9scpBSS9Fko8vqsi85yJTmQf1_5LgntItjV7MxIMAi9J7iinDTk876ahjBVFBNZZZBUWLxC55QyVtZY0NfLTHApsZRn6CLGPc6VN7UUb9EZwy1vaN2co-5mAmN7a3Syfix8X3zVJkGwfhr0Dopt9E4n6Ir1TtsxpmLj7JjZrvgJabDGunwvryHamPSYipukp-HReeONmWOxngPM8R1602sX4eKpr9Dv799uN5fl1a8f2836qjRMNKIULScMG9q1nMmWAlDSEQFa9hkhNdHiru25gAZLbQywWvCOSTAdycVayVbo08l3DL26N1Z5bY9959V9UOvr263iHFOWzwp9OXGn-e4AnYExBe3UFOxBh8ej8v_NaIfs86BqJuuWLgYfnwyC_zNDTOpgowHn9Ah-joqSVgrGZI5khfiJaoKPMUD_8gzB6pin2qslT7XkuYBEYZFlH_794ovoOT32F7u-noU</recordid><startdate>20190201</startdate><enddate>20190201</enddate><creator>Nasser, Ahmad</creator><creator>Azizian, Reza</creator><creator>Tabasi, Mohsen</creator><creator>Khezerloo, Jamil Kheirvari</creator><creator>Heravi, Fatemah Sadeghpour</creator><creator>Kalani, Morovat Taheri</creator><creator>Sadeghifard, Norkhoda</creator><creator>Amini, Razieh</creator><creator>Pakzad, Iraj</creator><creator>Radmanesh, Amin</creator><creator>Jalilian, Farid Azizi</creator><general>Korea Centers for Disease Control and Prevention</general><general>질병관리본부</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>ACYCR</scope></search><sort><creationdate>20190201</creationdate><title>Specification of Bacteriophage Isolated Against Clinical Methicillin-Resistant Staphylococcus Aureus</title><author>Nasser, Ahmad ; Azizian, Reza ; Tabasi, Mohsen ; Khezerloo, Jamil Kheirvari ; Heravi, Fatemah Sadeghpour ; Kalani, Morovat Taheri ; Sadeghifard, Norkhoda ; Amini, Razieh ; Pakzad, Iraj ; Radmanesh, Amin ; Jalilian, Farid Azizi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3575-584130c2d843982ee21d15ea9fd84161a5b8f45e709acce3654d39ecd11113893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Original</topic><topic>예방의학</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nasser, Ahmad</creatorcontrib><creatorcontrib>Azizian, Reza</creatorcontrib><creatorcontrib>Tabasi, Mohsen</creatorcontrib><creatorcontrib>Khezerloo, Jamil Kheirvari</creatorcontrib><creatorcontrib>Heravi, Fatemah Sadeghpour</creatorcontrib><creatorcontrib>Kalani, Morovat Taheri</creatorcontrib><creatorcontrib>Sadeghifard, Norkhoda</creatorcontrib><creatorcontrib>Amini, Razieh</creatorcontrib><creatorcontrib>Pakzad, Iraj</creatorcontrib><creatorcontrib>Radmanesh, Amin</creatorcontrib><creatorcontrib>Jalilian, Farid Azizi</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Korean Citation Index</collection><jtitle>Osong public health and research perspectives</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nasser, Ahmad</au><au>Azizian, Reza</au><au>Tabasi, Mohsen</au><au>Khezerloo, Jamil Kheirvari</au><au>Heravi, Fatemah Sadeghpour</au><au>Kalani, Morovat Taheri</au><au>Sadeghifard, Norkhoda</au><au>Amini, Razieh</au><au>Pakzad, Iraj</au><au>Radmanesh, Amin</au><au>Jalilian, Farid Azizi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specification of Bacteriophage Isolated Against Clinical Methicillin-Resistant Staphylococcus Aureus</atitle><jtitle>Osong public health and research perspectives</jtitle><addtitle>Osong Public Health Res Perspect</addtitle><date>2019-02-01</date><risdate>2019</risdate><volume>10</volume><issue>1</issue><spage>20</spage><epage>24</epage><pages>20-24</pages><issn>2210-9099</issn><eissn>2233-6052</eissn><abstract>The emergence of resistant bacteria is being increasingly reported around the world, potentially threatening millions of lives. Amongst resistant bacteria, methicillin-resistant (MRSA) is the most challenging to treat. This is due to emergent MRSA strains and less effective traditional antibiotic therapies to Staphylococcal infections. The use of bacteriophages (phages) against MRSA is a new, potential alternate therapy. In this study, morphology, genetic and protein structure of lytic phages against MRSA have been analysed. Isolation of livestock and sewage bacteriophages were performed using 0.4 μm membrane filters. Plaque assays were used to determine phage quantification by double layer agar method. Pure plaques were then amplified for further characterization. Sulfate-polyacrylamide gel electrophoresis and random amplification of polymorphic DNA were run for protein evaluation, and genotyping respectively. Transmission electron microscope was also used to detect the structure and taxonomic classification of phage visually. Head and tail morphology of bacteriophages against MRSA were identified by transmission electron microscopy and assigned to the family and the order. Bacteriophages are the most abundant microorganism on Earth and coexist with the bacterial population. They can destroy bacterial cells successfully and effectively. They cannot enter mammalian cells which saves the eukaryotic cells from lytic phage activity. In conclusion, phage therapy may have many potential applications in microbiology and human medicine with no side effect on eukaryotic cells.</abstract><cop>Korea (South)</cop><pub>Korea Centers for Disease Control and Prevention</pub><pmid>30847267</pmid><doi>10.24171/j.phrp.2019.10.1.05</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2210-9099
ispartof Osong Public Health and Research Perspectives, 2019, 10(1), , pp.20-24
issn 2210-9099
2233-6052
language eng
recordid cdi_nrf_kci_oai_kci_go_kr_ARTI_4402302
source PubMed Central (Training)
subjects Original
예방의학
title Specification of Bacteriophage Isolated Against Clinical Methicillin-Resistant Staphylococcus Aureus
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T07%3A58%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_nrf_k&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Specification%20of%20Bacteriophage%20Isolated%20Against%20Clinical%20Methicillin-Resistant%20Staphylococcus%20Aureus&rft.jtitle=Osong%20public%20health%20and%20research%20perspectives&rft.au=Nasser,%20Ahmad&rft.date=2019-02-01&rft.volume=10&rft.issue=1&rft.spage=20&rft.epage=24&rft.pages=20-24&rft.issn=2210-9099&rft.eissn=2233-6052&rft_id=info:doi/10.24171/j.phrp.2019.10.1.05&rft_dat=%3Cproquest_nrf_k%3E2189533923%3C/proquest_nrf_k%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c3575-584130c2d843982ee21d15ea9fd84161a5b8f45e709acce3654d39ecd11113893%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2189533923&rft_id=info:pmid/30847267&rfr_iscdi=true