Treadmill exercise with bone marrow stromal cells transplantation facilitates neuroprotective effect through BDNF-ERK1/2 pathway in spinal cord injury rats

• Published in: Journal of exercise rehabilitation 2018, 14(3), 55, pp.335-340
• Main Authors: Kim, You-Mi, Jin, Jun-Jang, Lee, Sam-Jun, Seo, Tae-Beom, Ji, Eun-Sang
• Format: Article
• Language: English
• Subjects: Original; 심리과학
• ISSN: 2288-176X; 2288-1778
• DOI: 10.12965/jer.1836264.132

Transplantation of bone marrow stromal cells (BMSCs) has been known as one of the effective therapeutic methods for functional recov-ery of spinal cord injury (SCI). Treadmill exercise also facilitates the functional recovery of SCI. Previously, we reported that combination of BMSCs transplantation with treadmill exercise potentiated the locomo-tor function in SCI rats. In the present study, we investigated whether recovery effect of BMSCs transplantation or treadmill exercise appears through the brain-derived neurotrophic factor (BDNF)-extracellular sig-nal–regulated kinases 1/2 (ERK1/2) pathway. The spinal cord contusion injury was performed at the T9-T10 level using the impactor. Cultured BMSCs were transplanted directly into the lesion 1 week after SCI. Treadmill exercise was performed 6 days per a week for 6 weeks. Western blot for Bax, Bcl-2, BDNF, tyrosine kinase B (TrkB), and phos-phorylated ERK1/2 (p-ERK1/2), phosphorylated JNK was performed. In the present results, combination of BMSCs transplantation with tread-mill exercise potently decreased Bax expression, potently increased Bcl-2 expression, and potently enhanced BDNF and TrkB expressions in the injured spinal cord. Combination of BMSCs transplantation with treadmill exercise further facilitated p-ERK1/2 and p-c-Jun expression levels. The present findings demonstrated the synergistic effect of treadmill exercise on neuroregenerative effect of BMSCs transplanta-tion appeared through the activation of BDNF-ERK1/2 pathway in SCI.