Honeybee (Apis cerana) major royal jelly protein 4 exhibits antimicrobial activity

Major royal jelly proteins (MRJPs) are important protein components of bee royal jelly (RJ) and exhibit various biological and pharmacological activities. The antimicrobial activities of the royalisin and the jelleines contained within MRJP 1 and MRJP 2 in RJ have been elucidated. However, the antim...

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Bibliographic Details
Published in:Journal of Asia-Pacific entomology 2019, 22(1), , pp.175-182
Main Authors: Kim, Bo Yeon, Lee, Kwang Sik, Jung, Boknam, Choi, Yong Soo, Kim, Hye Kyung, Yoon, Hyung Joo, Gui, Zhong-Zheng, Lee, Jungkwan, Jin, Byung Rae
Format: Article
Language:English
Subjects:
Antimicrobial activity
Apis cerana
Honeybee
Major royal jelly protein 4
Royal jelly
농수해양학
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Summary:Major royal jelly proteins (MRJPs) are important protein components of bee royal jelly (RJ) and exhibit various biological and pharmacological activities. The antimicrobial activities of the royalisin and the jelleines contained within MRJP 1 and MRJP 2 in RJ have been elucidated. However, the antimicrobial effects of other MRJPs remain largely unknown. In this study, we demonstrated the antimicrobial activity of the Asiatic honeybee (Apis cerana) MRJP 4 (AcMRJP4). Recombinant AcMRJP4 was expressed as a 63-kDa protein in baculovirus-infected insect cells. We examined the antimicrobial activity of recombinant AcMRJP4 against bacteria, fungi, and yeast. The mechanisms underlying the antimicrobial activity of AcMRJP4 were assessed using western blot analysis, immunofluorescence staining, and scanning electron microscopy. Recombinant AcMRJP4 bound to the cell walls of bacteria, fungi, and yeast and induced structural damage in the microbial cell walls. AcMRJP4 has an antimicrobial role and exhibits a broad spectrum of antimicrobial activities against bacteria, fungi, and yeast. We demonstrated that AcMRJP4 functions as an antimicrobial agent with activity against bacteria, fungi, and yeast. Together, our data identified a novel function of MRJP 4 as an antimicrobial agent. [Display omitted] •The cDNA of the honeybee (Apis cerana) major royal jelly protein 4 (AcMRJP4) was cloned.•AcMRJP4 exhibits antimicrobial activities against bacteria, fungi, and yeast.•AcMRJP4 functions as an antimicrobial agent.
ISSN:1226-8615
1876-7990
DOI:10.1016/j.aspen.2018.12.020