Growth behavior comparison of Listeria monocytogenes between Type strains and beef isolates in raw beef

• Published in: Food science and biotechnology 2018, 27(2), , pp.599-605
• Main Authors: Lee, So-Yeon, Kwon, Ki-Hyun, Chai, Changhoon, Oh, Se-Wook
• Format: Article
• Language: English
• Subjects: Beef; Chemistry; Chemistry and Materials Science; Food irradiation; Food processing industry; Food Science; Growth rate; Lag time; Listeria; Listeria monocytogenes; Nutrition; Pathogens; prediction; raw meat; specific growth rate; Storage; Storage temperature; 식품과학
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-017-0258-0

This study was conducted to compare the growth parameters of Listeria monocytogenes between beef isolates and Type strains in raw beef. Beef was artificially inoculated with 3 Log CFU/g levels and growth was measured during storage at various temperatures (5–25 °C) using conventional plating methods. The R 2 value for lag time ( λ ) and specific growth rate ( μ ) were determined using modified-Gompertz model, which were greater than 0.98 at all storage temperature except at 5 °C. B f , A f , and RMSE showed acceptable ranges, showed that the models are suitable for the modeling the growth of L. monocytogenes. At all temperatures, the λ of L. monocytogenes beef isolates was shorter than that of the L. monocytogenes Type strains, and the μ of beef isolates was higher than that of Type strains. These results showed that growth pattern prediction of beef inoculated with L . monocytogenes beef isolates gives more actual results than with Type strains.