Statistical optimization of culture medium for improved production of antimicrobial compound by Streptomyces rimosus AG-P1441

The nutritional requirements for antimicrobial activity of Streptomyces rimosus AG-P1441 were optimized using statistically-based experimental designs at a flask level. Based on a one-factor-at-a-time (OFAT) approach, glucose, corn starch and soybean meal were identified as the carbon and nitrogen s...

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Bibliographic Details
Published in:Food science and biotechnology 2018, 27(2), , pp.581-590
Main Authors: Ju, Yoonjung, Son, Kwang-Hee, Jin, Chunzhi, Hwang, Byung Soon, Park, Dong-Jin, Kim, Chang-Jin
Format: Article
Language:English
Subjects:
anti-infective agents
Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
antimicrobial properties
Bioreactors
carbon
Carbon sources
Chemistry
Chemistry and Materials Science
Corn
corn starch
culture media
experimental design
Fermentation
Food Science
Glucose
Magnesium chloride
nitrogen
Nitrogen sources
nutrient requirements
Nutrition
Nutritional requirements
Optimization
Pathogens
Phytophthora capsici
Productivity
Response surface methodology
soybean meal
Soybeans
Starch
Streptomyces rimosus
식품과학
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Summary:The nutritional requirements for antimicrobial activity of Streptomyces rimosus AG-P1441 were optimized using statistically-based experimental designs at a flask level. Based on a one-factor-at-a-time (OFAT) approach, glucose, corn starch and soybean meal were identified as the carbon and nitrogen sources having a significant effect on antimicrobial productivity. As a result of investigating the effect of glucose concentration, the highest antimicrobial activity was observed at 3% concentration. Response surface methodology (RSM) was then applied to optimize the growth medium components (corn starch, soybean meal, MgCl 2 and glutamate). Antimicrobial productivity increased sharply when the medium consisted of 3% glucose, 3.5% corn starch, 2.5% soybean meal, 1.2 mM MgCl 2 and 5.9 mM glutamate. The fermentation using optimized culture medium in a 5-L bioreactor allowed a significant increase in antimicrobial activity, evaluated by the paper disc assay, revealed a 29 mm inhibition zone diameter against Phytophthora capsici .
ISSN:1226-7708
2092-6456
DOI:10.1007/s10068-017-0257-1