Functionality of insect-cell-derived colorectal cancer vaccine candidate protein EpCAM-Fc in human dendritic cells

The baculovirus insect cell expression system is widely used to produce recom- binant proteins for various biomedical applications. Our previous study demon- strated that EpCAM, a colorectal cancer vaccine candidate protein, can be expressed in the baculovirus insect cell expression system. However,...

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Published in:Entomological research 2015, 45(3), , pp.162-166
Main Authors: Park, Y.S., Konkuk University School of Medicine, Seoul, Republic of Korea, Lee, J.H., Chung-Ang University, Seoul, Republic of Korea, Park, J.Y., Konkuk University, Seoul, Republic of Korea, Hwang, H.S., Konkuk University School of Medicine, Seoul, Republic of Korea, Zenke, M., RWTH Aachen University Medical School, Aachen, Germany, Han, D.W., Konkuk University School of Medicine, Seoul, Republic of Korea, Kang, Y.S., Konkuk University, Seoul, Republic of Korea, Ko, K., Chung-Ang University, Seoul, Republic of Korea, Ko, K., Konkuk University School of Medicine, Seoul, Republic of Korea
Format: Article
Language:English
Subjects:
BACULOVIRIDAE
baculovirus
blood
colorectal cancer
colorectal cancer,dendritic cells,EpCAM,human umbilical cord blood,insect cell expression system
colorectal neoplasms
dendritic cells
EpCAM
human umbilical cord blood
humans
immune response
immunotherapy
insect cell expression system
recombinant proteins
umbilical cord
vaccines
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Summary:The baculovirus insect cell expression system is widely used to produce recom- binant proteins for various biomedical applications. Our previous study demon- strated that EpCAM, a colorectal cancer vaccine candidate protein, can be expressed in the baculovirus insect cell expression system. However, its function- ality (the ability to elicit an immune response), which is important for its possible use as a colorectal cancer vaccine for immunotherapy, still needed to be confirmed. In this study, we examined the ability of recombinant EpCAM to induce maturation of immature dendritic cells (DCs) derived from CD34 + cells isolated from human umbilical cord blood. We demonstrated that EpCAM induces the expression of four DC maturation markers: CD80, CD83, CD86 and MHC II. These results suggest that EpCAM produced in the baculovirus insect cell expression system is func- tional in terms of its ability to trigger maturation of human DCs.
ISSN:1738-2297
1748-5967
1748-5967
DOI:10.1111/1748-5967.12109