Differential Expression of Gene Profiles in MRGX-treated Lung Cancer

Modified regular ginseng extract (MRGX) has stronger anti-cancer activity-possessing gensenoside profiles. To investigate changes in gene expression in the MRGX-treated lung cancer cells (A549), we examined genomic data with cDNA microarray results. After completing the gene-ontology-based analysis,...

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Bibliographic Details
Published in:Journal of pharmacopuncture 2013, 16(3), 47, pp.30-38
Main Authors: Kwon, Yong-Kyun, Lee, Seung-Yeul, Kang, Hwan-Soo, Sung, Jung-Suk, Cho, Chong-Kwan, Yoo, Hwa-Seung, Shin, Seungjin, Choi, Jong-Soon, Lee, Yeon-Weol, Jang, Ik-Soon
Format: Article
Language:English
Subjects:
gene ontology
lung cancer
microarray
modified regular ginseng extract (MRGX)
Original
한의학
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Summary:Modified regular ginseng extract (MRGX) has stronger anti-cancer activity-possessing gensenoside profiles. To investigate changes in gene expression in the MRGX-treated lung cancer cells (A549), we examined genomic data with cDNA microarray results. After completing the gene-ontology-based analysis, we grouped the genes into up-and down-regulated profiles and into ontology-related regulated genes and proteins through their interaction network. One hundred nine proteins that were up- and down-regulated by MRGX were queried by using IPA. IL8, MMP7 and PLAUR and were found to play a major role in the anti-cancer activity in MRGX-treated lung cancer cells. These results were validated using a Western blot analysis and a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. Most MRGX-responsive genes are up-regulated transiently in A549 cells, but down-regulated in a sustained manner in lung cancer cells.
ISSN:2093-6966
2234-6856
DOI:10.3831/KPI.2013.16.018