Characterization of biosurfactants as insecticidal metabolites produced by Bacillus subtilis Y9

Biosurfactants have been used for crop protection in integrated pest management programs, as they are generally biodegradable and environmentally friendly. While screening for insecticidal bacteria against green peach aphid, Myzus persicae, we found that a Bacillus isolate, Y9, exhibited strong surf...

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Bibliographic Details
Published in:Entomological research 2017, 47(1), , pp.55-59
Main Authors: Yang, Si Young, Lim, Da Jung, Noh, Mi Young, Kim, Jin Cheol, Kim, Young Cheol, Kim, In Seon
Format: Article
Language:English
Subjects:
aphid
Aphididae
Bacillus
Bacillus subtilis
Biodegradability
Biological control
biopesticide
biosurfactant
Biosurfactants
Chromatography
Column chromatography
Data processing
High-performance liquid chromatography
integrated pest management
Isomers
Leucine
Metabolites
Myzus persicae
organic farming
Pest control
Plant protection
Prunus
Surfactants
Surfactin
생물학
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Summary:Biosurfactants have been used for crop protection in integrated pest management programs, as they are generally biodegradable and environmentally friendly. While screening for insecticidal bacteria against green peach aphid, Myzus persicae, we found that a Bacillus isolate, Y9, exhibited strong surface tension activity and produced insecticidal metabolites. The insecticidal metabolites with surface tension activity were extracted from the cell‐free supernatant of Y9 and subjected to repetitive column chromatography and preparative high performance liquid chromatography (HPLC). Based on data from instrumental analyses, the metabolites were identified as surfactin isomers consisting of C14[Leu7], C14[Val7] and C15[Leu7]. The isomers with leucine showed higher insecticidal activity than the isomers with valine. Y9 was capable of producing biosurfactants as insecticidal metabolites against M. persicae, and showed potential for use as a biocontrol agent in an integrated pest management program.
ISSN:1738-2297
1748-5967
1748-5967
DOI:10.1111/1748-5967.12200