Terrabacter ginsengisoli sp. nov., isolated from ginseng cultivating soil

A Gram-positive, strictly aerobic, nonmotile, yellowish, coccus-rod-shaped bacterium (designated Gsoil 653 T ) isolated from ginseng cultivating soil was characterized using a polyphasic approach to clarify its taxonomic position. The strain Gsoil 653 T exhibited optimal growth at pH 7.0 on R2A agar...

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Bibliographic Details
Published in:The journal of microbiology 2018, 56(5), , pp.331-336
Main Authors: Jin, Mei-Fang, Quan, Xiao-Tian, Siddiqi, Muhammad Zubair, Liu, Qing-Zhen, Yu, Hong-Shan, Im, Wan-Taek
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Carbon 14
Carbon isotopes
Cardiolipin
Deoxyribonucleic acid
DNA
Ethanolamine
Fatty acids
Ginseng
Life Sciences
Lipids
Microbial Systematics and Evolutionary Microbiology
Microbiology
New species
Nucleotide sequence
Phosphatidylglycerol
Phylogeny
Quinones
rRNA 16S
Soil
생물학
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Summary:A Gram-positive, strictly aerobic, nonmotile, yellowish, coccus-rod-shaped bacterium (designated Gsoil 653 T ) isolated from ginseng cultivating soil was characterized using a polyphasic approach to clarify its taxonomic position. The strain Gsoil 653 T exhibited optimal growth at pH 7.0 on R2A agar medium at 30°C. Phylogenetic analysis based on 16S rRNA gene sequence similarities, indicated that Gsoil 653 T belongs to the genus Terrabacter of the family Humibacillus , and was closely related to Terrabacter tumescens DSM 20308 T (98.9%), Terrabacter carboxydivorans PY2 T (98.9%), Terrabacter terrigena ON10 T (98.8%), Terrabacter terrae PPLB T (98.6%), and Terrabacter lapilli LR-26 T (98.6%). The DNA G + C content was 70.5 mol%. The major quinone was MK-8(H 4 ). The primary polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidyl-ethanolamine. The predominant fatty acids were iso-C 15:0 , iso-C 16:0 , iso-C 14:0 , and anteiso-C 15:0 , as in the case of genus Terrabacter , thereby supporting the categorization of strain Gsoil 653 T . However, the DNA-DNA relatedness between Gsoil 653 T and closely related strains of Terrabacter species was low at less than 31%. Moreover, strain Gsoil 653 T could be both genotypically and phenotypically distinguished from the recognized species of the genus Terrabacter . This isolate, therefore, represents a novel species, for which the name Terrabacter ginsengisoli sp. nov. is proposed with the type strain Gsoil 653 T (= KACC 19444 T = LMG 30325 T ).
ISSN:1225-8873
1976-3794
DOI:10.1007/s12275-018-8098-z