유세포 분석기와 Neutral Red Retention Assay를 이용한 자연산 참굴(Crassostrea gigas)의 면역력 측정

Hemocyte parameters of the wild Pacific oyster Crassostrea gigas inhabiting intertidal zones in small bays (Gwangyang and Jinhae Bay) on the southern coast of Korea were evaluated using flow cytometry and neutral red retention (NRR) assay. Morphological features, cell count, mortality, DNA damage, p...

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Bibliographic Details
Published in:Ocean and polar research 2012, 34(2), , pp.137-149
Main Authors: 홍현기, 강현실, 김영옥, 최광식, Hong, Hyun-Ki, Kang, Hyun-Sil, Kim, Young-Ok, Choi, Kwang-Sik
Format: Article
Language:Korean
Subjects:
해양학
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Summary:Hemocyte parameters of the wild Pacific oyster Crassostrea gigas inhabiting intertidal zones in small bays (Gwangyang and Jinhae Bay) on the southern coast of Korea were evaluated using flow cytometry and neutral red retention (NRR) assay. Morphological features, cell count, mortality, DNA damage, phagocytosis, and lysosomal membrane stability of hemocytes were analyzed. Three types of hemocytes were identified in the oyster hemolymph: granulocytes, hyalinocytes, and blast-like cells. Immune related functions of hemocyte including phagocytosis and lysosomal membrane stability were significantly different among the study areas (P
ISSN:1598-141X
2234-7313
DOI:10.4217/OPR.2012.34.2.137