Carbonic anhydrase I in a cartilaginous fish, the shortspine spurdog (Squalus mitsukurii)

Carbonic anhydrase (CA), a ubiquitous enzyme found in many species, including fishes, is involved in physiological functions such as pH homeostasis, calcification, photosynthesis, and ionic regulation. CA I, a member of the α-CA family, is a cytoplasmic isozyme involved in carbon dioxide transport,...

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Bibliographic Details
Published in:Ocean science journal 2016, 51(3), , pp.373-379
Main Authors: Kim, Soo Cheol, Sumi, Kanij Rukshana, Kim, Jung Woo, Choi, Myeong Rak, Min, Byung Hwa, Kho, Kang Hee
Format: Article
Language:English
Subjects:
Analytical methods
Aquatic Pollution
Biological data
Calcification
Carbon dioxide
Carbonic anhydrase
Earth and Environmental Science
Earth Sciences
Fish
Fisheries
Gel electrophoresis
Gels
Isoenzymes
Marine & Freshwater Sciences
Marine fishes
Oceanography
Photosynthesis
Physicochemical processes
Physicochemical properties
Physiological functions
Sharks
Sodium
Squalus mitsukurii
Taxa
Tissue
Tissues
Waste Water Technology
Water Management
Water Pollution Control
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Summary:Carbonic anhydrase (CA), a ubiquitous enzyme found in many species, including fishes, is involved in physiological functions such as pH homeostasis, calcification, photosynthesis, and ionic regulation. CA I, a member of the α-CA family, is a cytoplasmic isozyme involved in carbon dioxide transport, ion exchange, and acid-base balance. Approximately half of the extant shark species occur only in deep waters; however, few published studies on sharks include these taxa. As fisheries worldwide enter deeper waters, the provision of biological data for these little-known taxa is critical to their management and conservation. To address this limitation, we aimed to detect CA I in various tissues of the shortspine spurdog ( Squalus mitsukurii ) and characterize its physicochemical properties by using sodium dodecyl-sulfate polyacrylamide gel electrophoresis and isoelectric focusing, together with immunohistochemistry. CA I was detected on SDS-PAGE and western blot analysis as a specific band at 29 kDa in various tissues of the shortspine spurdog, and as a specific band at pI 6.5 in various tissues of the shortspine spurdog by IEF and western blot analysis. CA I immunoreactivity in various tissues of the shortspine spurdog was detected in intracellular locations. To our knowledge, this is the first report of the localization of CA isozymes in various tissues of S. mitsukurii .
ISSN:1738-5261
2005-7172
DOI:10.1007/s12601-016-0033-9