Knock out of the annexin gene OsAnn3 via CRISPR/Cas9-mediated genome editing decreased cold tolerance in rice

• Published in: Journal of plant biology = Singmul Hakhoe chi 2017, 60(6), , pp.539-547
• Main Authors: Shen, Chunxiu, Que, Zhiqun, Xia, Yumei, Tang, Ning, Li, Ding, He, Ronghua, Cao, Mengliang
• Format: Article
• Language: English
• Subjects: Abiotic stress; Annexins; Biomedical and Life Sciences; Calcium; Calcium ions; Cloning; Cold; Cold tolerance; Cold treatment; CRISPR; Drought; Editing; Electrical conductivity; Electrical resistivity; Environmental stress; Genes; Genomes; Kinases; Life Sciences; Mutants; Original Article; Phenotypes; Phospholipids; Plant Breeding/Biotechnology; Plant Ecology; Plant Genetics and Genomics; Plant Sciences; Plant Systematics/Taxonomy/Biogeography; Plantlets; Proteins; Rice; Transcription factors; 생물학
• ISSN: 1226-9239; 1867-0725
• DOI: 10.1007/s12374-016-0400-1

Plant annexins are Ca 2+ -dependent phospholipid-binding proteins and exist as multigene families in plants. They are implicated in the regulation of plant development as well as protection from environmental stresses. In this study, the rice annexin gene OsAnn3 knockout was performed via the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated proteins) mediated genome editing. Thus, mutant plantlets were successfully obtained. We identified cold tolerance phenotype of T 1 mutant lines from T0 biallelic mutants using the 4∼6°C for 3 days cold treatment. The results showed that REC (the relative electrical conductivity) of T 1 mutant lines was increased, and the survival ratio of T 1 mutant lines was decreased dramatically compared with the wild type after the exposure to cold treatment. It was suggested that OsAnn3 was involved in cold tolerance of rice.