Three P5CS genes including a novel one from Lilium regale play distinct roles in osmotic, drought and salt stress tolerance

Proline accumulations in abiotically stressed plants is generally considered to benefit their stress tolerance. The Δ 1 -Pyrroline-5-carboxylate synthetase ( P5CS ) gene family, which encodes the rate-limiting enzyme in proline biosynthesis pathway, usually contains two duplicated genes in most plan...

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Bibliographic Details
Published in:Journal of plant biology = Singmul Hakhoe chi 2016, 59(5), , pp.456-466
Main Authors: Wei, Chi, Cui, Qi, Zhang, Xi-Qing, Zhao, Yu-Qian, Jia, Gui-Xia
Format: Article
Language:English
Subjects:
Abiotic stress
Amino acids
Biomedical and Life Sciences
Biosynthesis
Drought
E coli
Elongation
Gene duplication
Genes
Life Sciences
Lilium regale
Original Article
Osmotic stress
Phylogeny
Plant Breeding/Biotechnology
Plant Ecology
Plant Genetics and Genomics
Plant Sciences
Plant Systematics/Taxonomy/Biogeography
Proline
Roots
Salinity tolerance
Salt tolerance
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Summary:Proline accumulations in abiotically stressed plants is generally considered to benefit their stress tolerance. The Δ 1 -Pyrroline-5-carboxylate synthetase ( P5CS ) gene family, which encodes the rate-limiting enzyme in proline biosynthesis pathway, usually contains two duplicated genes in most plants. However, three P5CS genes including LrP5CS1 , LrP5CS2 as well as a third one, LrP5CS3 , were isolated from Lilium regale . LrP5CS3 is highly identical to LrP5CS1 in amino acid sequences, indicating they could come from a paralogous duplication. The phylogenetic tree suggested that the duplication of LrP5CS occurred independently after the divergence of Liliales and commelinoids. The expression of LrP5CS1 was strongly induced in leaves and roots both under drought and salinity, while that of LrP5CS3 was upregulated more moderately. LrP5CS2 stayed almost constitutive under stress. LrP5CS1 exhibited the highest activity after expressed in E. coli . Overexpression of LrP5CS genes conferred enhanced osmotic, drought and salt tolerance on transgenic Arabidopsis without negative effects in unstressed condition. Under salt stress, lines LrP5CS2 accumulated fewer proline than others, and lines LrP5CS1 grew better in root elongation. The roots of lines LrP5CS3 grew better than all others under unstressed condition and osmotic stress. Our study suggests that the three LrP5CS genes play distinct roles respectively in proline accumulation and abiotic stress tolerance.
ISSN:1226-9239
1867-0725
DOI:10.1007/s12374-016-0189-y