Map-based cloning and functional analysis of the chromogen gene C in rice (Oryza sativa L.)

The chromogen gene C is critical for anthocyanin regulation in rice, and apiculus color is an important agronomic trait in selective breeding and variety purification. Mapbased cloning and in-depth functional analysis of the C gene will be useful for understanding the molecular mechanism of anthocya...

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Bibliographic Details
Published in:Journal of plant biology = Singmul Hakhoe chi 2016, 59(5), , pp.496-505
Main Authors: Zhao, Shasha, Wang, Cuihong, Ma, Jian, Wang, Shuai, Tian, Peng, Wang, Jiulin, Cheng, Zhijun, Zhang, Xin, Guo, Xiuping, Lei, Cailin
Format: Article
Language:English
Subjects:
Anthocyanins
Biomedical and Life Sciences
Biosynthesis
C gene
Chromosome 6
Cloning
Color
Cultivars
Functional analysis
Genetic analysis
Life Sciences
Molecular modelling
Original Article
Oryza sativa japonica
Plant breeding
Plant Breeding/Biotechnology
Plant Ecology
Plant Genetics and Genomics
Plant Sciences
Plant Systematics/Taxonomy/Biogeography
Regulatory mechanisms (biology)
Rice
Selective breeding
Stigma
생물학
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Summary:The chromogen gene C is critical for anthocyanin regulation in rice, and apiculus color is an important agronomic trait in selective breeding and variety purification. Mapbased cloning and in-depth functional analysis of the C gene will be useful for understanding the molecular mechanism of anthocyanin biosynthesis and for rice breeding. Japonica landrace Lijiangxintuanheigu (LTH) has red apiculi and purple stigmas. Genetic analysis showed that red apiculus and purple stigma in LTH co-segregated indicating control by a single dominant gene, or by two completely linked genes. Using 1,851 recessive individuals from two F 2 populations, the target gene OsC was delimited to a 70.8 kb interval on chromosome 6 that contains the rice homologue of the maize anthocyanin regulatory gene C1 . When the entire OsC gene and its full-length cDNA cloned from LTH were transformed into japonica cultivar Kitaake with colorless apiculi and stigmas all positive transformants had red apiculi but non-colored stigmas, validating that OsC alone was responsible for the apiculus color and represented the functional C gene. OsC was constitutively expressed in all tissues examined, with strongest expression in leaf blades. These results set a foundation to clarify the regulatory mechanisms of OsC in the anthocyanin biosynthetic pathway.
ISSN:1226-9239
1867-0725
DOI:10.1007/s12374-016-0227-9