Cyanobacterial biodiversity of semiarid public drinking water supply reservoirs assessed via next-generation DNA sequencing technology

• Published in: The journal of microbiology 2019, 57(6), , pp.450-460
• Main Authors: Lorenzi, Adriana Sturion, Chia, Mathias Ahii, Lopes, Fabyano Alvares Cardoso, Silva, Genivaldo Gueiros Z., Edwards, Robert A., Bittencourt-Oliveira, Maria do Carmo
• Format: Article
• Language: English
• Subjects: Bacterial Toxins - analysis; Bacterial Toxins - genetics; Bacterial Toxins - isolation & purification; Biodiversity; Biomedical and Life Sciences; Brazil; Composition; Cyanobacteria; Cyanobacteria - classification; Cyanobacteria - genetics; Cyanobacteria - isolation & purification; Data; Deoxyribonucleic acid; DNA; DNA sequences; DNA sequencing; DNA, Bacterial - analysis; Drinking water; Drinking Water - microbiology; Dry season; ELISA; Environmental Monitoring - methods; Enzyme-linked immunosorbent assay; Genera; Genes, Bacterial - genetics; High-Throughput Nucleotide Sequencing - methods; Life Sciences; Metagenomics - methods; Microbial Ecology and Environmental Microbiology; Microbiology; Microcystins; Microcystis; Microcystis - genetics; Rainy season; Reservoirs; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 23S - genetics; rRNA 16S; rRNA 23S; Saxitoxin - genetics; Seasons; Sequence Analysis, DNA - methods; Sequencing; Taxonomy; Technology; Toxins; Uracil - analogs & derivatives; Water Microbiology; Water Supply; Wet season; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-019-8349-7

Next-generation DNA sequencing technology was applied to generate molecular data from semiarid reservoirs during well-defined seasons. Target sequences of 16S-23S rRNA ITS and cpc BA-IGS were used to reveal the taxonomic groups of cyanobacteria present in the samples, and genes coding for cyanotoxins such as microcystins ( mcy E), saxitoxins ( sxt A), and cylindrospermopsins ( cyr J) were investigated. The presence of saxitoxins in the environmental samples was evaluated using ELISA kit. Taxonomic analyses of high-throughput DNA sequencing data showed the dominance of the genus Microcystis in Mundaú reservoir. Furthermore, it was the most abundant genus in the dry season in Ingazeira reservoir. In the rainy season, 16S-23S rRNA ITS analysis revealed that Cylindrospermopsis raciborskii comprised 46.8% of the cyanobacterial community in Ingazeira reservoir, while the cpcBAIGS region revealed that C. raciborskii (31.8%) was the most abundant taxon followed by Sphaerospermopsis aphanizomenoides (17.3%) and Planktothrix zahidii (16.6%). Despite the presence of other potential toxin-producing genera, the detected sxt A gene belonged to C. raciborskii , while the mcy E gene belonged to Microcystis in both reservoirs. The detected mcy E gene had good correlation with MC content, while the amplification of the sxt A gene was related to the presence of STX. The cyr J gene was not detected in these samples. Using DNA analyses, our results showed that the cyanobacterial composition of Mundaú reservoir was similar in successive dry seasons, and it varied between seasons in Ingazeira reservoir. In addition, our data suggest that some biases of analysis influenced the cyanobacterial communities seen in the NGS output of Ingazeira reservoir.