DNA methylation and expression patterns of key tissue-specific genes in adult stem cells and stomach tissues

CpG-island margins and non-island-CpG sites round the transcription start sites of CpG-island-positive and -negative genes are methylated to various degrees in a tissue-specific manner. These methylation-variable CpG sites were analyzed to delineate a relationship between the methylation and transcr...

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Bibliographic Details
Published in:Journal of Korean medical science 2009, 24(5), 132, pp.918-929
Main Authors: Hong, Seung-Jin, Kang, Moo-Il, Oh, Jung-Hwan, Jung, Yu-Chae, Kim, Young-Ho, Kim, Sung-Ja, Choi, Seung-Hye, Seo, Eun-Joo, Choi, Sang-Wook, Rhyu, Mun-Gan
Format: Article
Language:English
Subjects:
Adipose Tissue - cytology
Adolescent
Adult
Adult Stem Cells - cytology
Adult Stem Cells - metabolism
Aged
CpG Islands - genetics
DNA Methylation
Female
Gene Expression Profiling
Humans
Male
Middle Aged
Original
Polymerase Chain Reaction
Stomach - cytology
Stomach - metabolism
Stromal Cells - metabolism
Transcription Initiation Site
Transcription, Genetic
의학일반
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Summary:CpG-island margins and non-island-CpG sites round the transcription start sites of CpG-island-positive and -negative genes are methylated to various degrees in a tissue-specific manner. These methylation-variable CpG sites were analyzed to delineate a relationship between the methylation and transcription of the tissue-specific genes. The level of tissue-specific transcription was estimated by counting the number of the total transcripts in the SAGE (serial analysis of gene expression) database. The methylation status of 12 CpG-island margins and 21 non-island CpG sites near the key tissue-specific genes was examined in pluripotent stromal cells obtained from fat and bone marrow samples as well as in lineage-committed cells from marrow bulk, stomach, colon, breast, and thyroid samples. Of the 33 CpG sites examined, 10 non-island-CpG sites, but none of the CpG-island margins were undermethylated concurrent with tissue-specific expression of their nearby genes. The net methylation of the 33 CpG sites and the net amount of non-island-CpG gene transcripts were high in stomach tissues and low in stromal cells. The present findings suggest that the methylation of the non-island-CpG sites is inversely associated with the expression of the nearby genes, and the concert effect of transitional-CpG methylation is linearly associated with the stomach-specific genes lacking CpG-islands.
ISSN:1011-8934
1598-6357
DOI:10.3346/jkms.2009.24.5.918