Hypermethylation of p16 INK4a in Korean Non-small Cell Lung CancerPatients

Promoter hypermethylation of the p16INK4a gene was investigated in 81 sets of samples of tumor tissue and adjacent normal tissue from Korean patients with primary lung cancer, using the modified real-time polymerase chain reaction (PCR)/ SYBR Green detection method. The results showed hypermethylati...

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Bibliographic Details
Published in:Journal of Korean medical science 2007, 22(S), , pp.32-37
Main Authors: Young-Seoub Hong, 노미숙, Na-Young Kim, Hye-Jung Lee, Hee-Kyoung Kim, Kyung-Eun Lee, 곽종영, 김준연
Format: Article
Language:English
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의학일반
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Summary:Promoter hypermethylation of the p16INK4a gene was investigated in 81 sets of samples of tumor tissue and adjacent normal tissue from Korean patients with primary lung cancer, using the modified real-time polymerase chain reaction (PCR)/ SYBR Green detection method. The results showed hypermethylation of p16INK4a in 27.2% of tumor tissues, and in 11.1% of adjacent normal tissue. No significant association was found between the overall aberrant methylation in tumor and corresponding normal specimens (r=0.137, p=0.219). In 22 cases with p16INK4a hypermethylation in tumor tissues, only 4 (18.1%) cases were found to have a hypermethylated normal tissue specimen. The findings of this study show that smoking can influence the methylation level of the promoter region of p16INK4a, and that this occurs in tumor tissues more frequently than in normal tissues. Other clinicopathological characteristics, including age, sex, tumor stage, and histologic type were not found to be correlated with p16INK4a methylation. KCI Citation Count: 8
ISSN:1011-8934
1598-6357