Arthrobacter dokdonellae sp. nov., isolated from a plant of the genus Campanula

• Published in: The journal of microbiology 2019, 57(9), , pp.732-737
• Main Authors: Koh, Hyeon-Woo, Kang, Myung-Suk, Lee, Ki-Eun, Lee, Eun-Young, Kim, Hongik, Park, Soo-Je
• Format: Article
• Language: English
• Subjects: Arthrobacter; Arthrobacter - classification; Arthrobacter - genetics; Arthrobacter - isolation & purification; Arthrobacter - metabolism; Base Composition; Biomedical and Life Sciences; Campanula; Campanulaceae - microbiology; Cardiolipin; Catalase; Cell Wall - genetics; Cell Wall - metabolism; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Fatty acids; Fatty Acids - chemistry; Fatty Acids - metabolism; Gram stain; Indigenous plants; Life Sciences; Lipids; Microbial Systematics and Evolutionary Microbiology; Microbiological strains; Microbiology; New species; Nucleotide sequence; Peptidoglycan - chemistry; Peptidoglycan - metabolism; Peptidoglycans; Phosphatidylglycerol; Phosphatidylinositol; Phylogeny; Quinones; Republic of Korea; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequencing; Sodium chloride; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-019-8540-x

A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain, designated as DCT-5 T , was isolated from a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea. Growth of the strain DCT-5 T was observed at 15–37°C (optimum 30°C) on R2A broth, pH 6.0–8.0 (optimum 7.0), and 0–5% (w/v) NaCl concentration (optimum 0%). The 16S rRNA gene sequence analysis revealed that strain DCT-5 T was most closely related to Arthrobacter silviterrae KIS14-16 T , Arthrobacter livingstonensis LI2 T , Arthrobacter stackebrandtii CCM 2783 T , Arthrobacter cryoconiti Cr6-08 T , Arthrobacter ramosus CCM 1646 T , and Arthrobacter psychrochitiniphilus GP3 T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively. The DNA G+C content of strain DCT-5 T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16 T , A. livingstonensis LI2 T , A. stackebrandtii CCM 2783 T , A. psychrochitiniphilus GP3 T , A. ramosus CCM 1646 T , and A. cryoconiti Cr6-08 T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively. The major quinone was MK-9(H 2 ) and major fatty acids were anteiso-C 15:0 , anteiso-C 17:0 , iso-C 15:0 , and iso-C 16:0 . The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), unidentified glycolipid (GL), two unidentified aminophospholipids (APLs), and three unidentified lipids (Ls). The peptidoglycan type was A3α. On the basis of phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics, strain DCT-5 T represents a novel species of the genus Arthrobacter , for which the name Arthrobacter dokdonellae sp. nov. is proposed. The type strain is DCT-5 T (= KCTC 49189 T = LMG 31284 T ).