The Role of Rice Vacuolar Invertase2 in Seed Size Control

Sink strength optimizes sucrose import, which is fundamental to support developing seed grains and increase crop yields, including those of rice ( ). In this regard, little is known about the function of vacuolar invertase (VIN) in controlling sink strength and thereby seed size. Here, in rice we an...

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Bibliographic Details
Published in:Molecules and cells 2019, 42(10), , pp.711-720
Main Authors: Lee, Dae-Woo, Lee, Sang-Kyu, Rahman, Md Mizanor, Kim, Yu-Jin, Zhang, Dabing, Jeon, Jong-Seong
Format: Article
Language:English
Subjects:
beta-Fructofuranosidase - metabolism
CRISPR-Associated Protein 9 - metabolism
CRISPR-Cas Systems - genetics
Metabolome
Mutation - genetics
Organ Size
Oryza - enzymology
Photosynthesis
Plants, Genetically Modified
Seeds - anatomy & histology
Seeds - enzymology
Starch - metabolism
Sucrose - metabolism
Vacuoles - enzymology
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Summary:Sink strength optimizes sucrose import, which is fundamental to support developing seed grains and increase crop yields, including those of rice ( ). In this regard, little is known about the function of vacuolar invertase (VIN) in controlling sink strength and thereby seed size. Here, in rice we analyzed mutants of two VINs, OsVIN1 and OsVIN2, to examine their role during seed development. In a phenotypic analysis of the T-DNA insertion mutants, only the OsVIN2 mutant exhibited reduced seed size and grain weight. Scanning electron microscopy analysis revealed that the small seed grains of can be attributed to a reduction in spikelet size. A significant decrease in VIN activity and hexose level in the spikelets interfered with spikelet growth. In addition, significant reduction in starch and increase in sucrose, which are characteristic features of reduced turnover and flux of sucrose due to impaired sink strength, were evident in the pre-storage stage of developing grains. In situ hybridization analysis found that expression of was predominant in the endocarp of developing grains. A genetically complemented line with a native genomic clone of rescued reduced VIN activity and seed size. Two additional mutants, and generated by the CRISPR/Cas9 method, exhibited phenotypes similar to those of in spikelet and seed size, VIN activity, and sugar metabolites. These results clearly demonstrate an important role of OsVIN2 as sink strength modulator that is critical for the maintenance of sucrose flux into developing seed grains.
ISSN:1016-8478
0219-1032
DOI:10.14348/molcells.2019.0109