RRM but not the Asp/Glu domain of hnRNP C1/C2 is required for splicing regulation of Ron exon 11 pre-mRNA

The Ron proto-oncogene is a human receptor for macrophage-stimulating protein (MSP). The exclusion of exon 11 in alternative splicing generates ΔRON protein that is constitutively activated. Heterogenous ribonucleaoprotein (hnRNP) C 1 /C 2 is one of the most abundant proteins in cells. In this manus...

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Published in:BMB reports 2019, 52(11), , pp.641-646
Main Authors: Moon, Heegyum, Jang, Ha Na, Liu, Yongchao, Choi, Namjeong, Oh, Jagyeong, Ha, Jiyeon, Kim, Hyeon Ho, Zheng, Xuexiu, Shen, Haihong
Format: Article
Language:English
Subjects:
화학
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Summary:The Ron proto-oncogene is a human receptor for macrophage-stimulating protein (MSP). The exclusion of exon 11 in alternative splicing generates ΔRON protein that is constitutively activated. Heterogenous ribonucleaoprotein (hnRNP) C 1 /C 2 is one of the most abundant proteins in cells. In this manuscript, we showed that both hnRNP C 1 and C 2 promoted exon 11 inclusion of Ron pre-mRNA and that hnRNP C 1 and hnRNP C 2 functioned independently but not cooperatively. Moreover, hnRNP C 1 stimulated exon 11 splicing through intron 10 activation but not through intron 11 splicing. Furthermore, we showed that, whereas the RRM domain was required for hnRNP C 1 function, the Asp/Glu domain was not. In conclusion, hnRNP C 1 /C 2 promoted exon 11 splicing independently by stimulating intron 10 splicing through RRM but not through the Asp/Glu domain.
ISSN:1976-6696
1976-670X
DOI:10.5483/BMBRep.2019.52.11.080