Human B1 Cells are the Main Blood Group A-Specific B Cells That Have a Moderate Correlation With Anti-A Antibody Titer

• Published in: Annals of laboratory medicine 2020, 40(1), , pp.48-56
• Main Authors: Xu, Yixuan, Lee, Jae Ghi, Yan, Ji Jing, Ryu, Jung Hwa, Xu, Songji, Yang, Jaeseok
• Format: Article
• Language: English
• Subjects: ABO Blood-Group System - immunology; Adult; Antibodies - blood; Antigens, CD1 - metabolism; B-Lymphocyte Subsets - cytology; B-Lymphocyte Subsets - metabolism; B-Lymphocytes - cytology; B-Lymphocytes - metabolism; Female; Glycoproteins - metabolism; Humans; Kidney Failure, Chronic - immunology; Kidney Failure, Chronic - pathology; Leukosialin - metabolism; Male; Middle Aged; Original; Peritoneal Dialysis; Prospective Studies; 병리학
• ISSN: 2234-3806; 2234-3814
• DOI: 10.3343/alm.2020.40.1.48

Anti-carbohydrate antibody responses, including those of anti-blood group ABO antibodies, are yet to be thoroughly studied in humans. Because anti-ABO antibody-mediated rejection is a key hurdle in ABO-incompatible transplantation, it is important to understand the cellular mechanism of anti-ABO responses. We aimed to identify the main human B cell subsets that produce anti-ABO antibodies by analyzing the correlation between B cell subsets and anti-ABO antibody titers. Blood group A-binding B cells were analyzed in peritoneal fluid and peripheral blood samples from 43 patients undergoing peritoneal dialysis and 18 healthy volunteers with blood group B or O. The correlation between each blood group A-specific B cell subset and anti-A antibody titer was then analyzed using Pearson's correlation analysis. Blood group A-binding B cells were enriched in CD27 CD43 CD1c B1, CD5 B1, CD11b B1, and CD27 CD43 CD1c marginal zone-B1 cells in peripheral blood. Blood group A-specific B1 cells ( =0.029 and R=0.356 for IgM; =0.049 and R=0.325 for IgG) and marginal zone-B1 cells ( =0.011 and R=0.410 for IgM) were positively correlated with anti-A antibody titer. Further analysis of peritoneal B cells confirmed B1 cell enrichment in the peritoneal cavity but showed no difference in blood group A-specific B1 cell enrichment between the peritoneal cavity and peripheral blood. Human B1 cells are the key blood group A-specific B cells that have a moderate correlation with anti-A antibody titer and therefore constitute a potential therapeutic target for successful ABO-incompatible transplantation.