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Temporal Regulation of Ovine Interferon-tau Gene by the Transcription Factor Eomesodermin in the Peri-Implantation Period
Interferon tau (IFNT) regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, is essential for the maintenance of early pregnancy, but a definitive mechanism for its temporal transcription has not been elucidated. We and others have observed the T-box protein eomesod...
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Published in: | Journal of animal reproduction & biotechnology (Online) 2019, 34(4), , pp.292-299 |
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creator | Kim, Min-Su Lim, Hyun-Joo Lee, Ji Hwan Hur, Tae Young Son, Jun Kyu |
description | Interferon tau (IFNT) regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, is essential for the maintenance of early pregnancy, but a definitive mechanism for its temporal transcription has not been elucidated. We and others have observed the T-box protein eomesodermin (EOMES) exhibited high mRNA expression in the ovine embryonic trophectoderm; thus, both caudal-relatedhomeobox-2 (CDX2) and EOMES coexist during the early stages of conceptus development. Objective of this study was to examine the effect of EOMES on ovine IFNT gene transcription when evaluated with CDX2, ETS2 and AP1 transcription factors implicated in the control of cell differentiation in the trophectoderm. In this study, quantitatively via reverse transcription-polymerase chain reaction (RT-PCR) analysis between ovine trophoblast cells was initially performed, finding that transcription factors CDX2 and ‘EOMES transcription factor mRNAs’ were specific to trophectoderm cells. These mRNAs were also found in days 15, 17, and 21 ovine conceptuses. Furthermore, human choriocarcinoma JEG3 cells (trophoblast cell line) were cotransfected with an ovine IFNT (-654bp)-luciferase reporter (-654-oIFNT-Luc) construct and several transcription factor expression plasmids. Cotransfection of the reporter construct with CDX2, ETS2 and AP1 increased transcription of -654-oIFNT-Luc by about 11-fold compared with transfection of the construct alone. When cells were initially transfected with EOMES followed by transfection with CDX2, ETS2 and/or AP1, the expression of -654-oIFNT-Luc was decreased. Also, EOMES factor inhibited the stimulatory activity of CDX2 alone. These results suggest that when conceptuses attach to the uterine epithelium, ovine IFNT gene transcription is down-regulated by an increase of EOMES factor expression in the attached ovine trophoblast cells. |
doi_str_mv | 10.12750/JARB.34.4.292 |
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We and others have observed the T-box protein eomesodermin (EOMES) exhibited high mRNA expression in the ovine embryonic trophectoderm; thus, both caudal-relatedhomeobox-2 (CDX2) and EOMES coexist during the early stages of conceptus development. Objective of this study was to examine the effect of EOMES on ovine IFNT gene transcription when evaluated with CDX2, ETS2 and AP1 transcription factors implicated in the control of cell differentiation in the trophectoderm. In this study, quantitatively via reverse transcription-polymerase chain reaction (RT-PCR) analysis between ovine trophoblast cells was initially performed, finding that transcription factors CDX2 and ‘EOMES transcription factor mRNAs’ were specific to trophectoderm cells. These mRNAs were also found in days 15, 17, and 21 ovine conceptuses. Furthermore, human choriocarcinoma JEG3 cells (trophoblast cell line) were cotransfected with an ovine IFNT (-654bp)-luciferase reporter (-654-oIFNT-Luc) construct and several transcription factor expression plasmids. Cotransfection of the reporter construct with CDX2, ETS2 and AP1 increased transcription of -654-oIFNT-Luc by about 11-fold compared with transfection of the construct alone. When cells were initially transfected with EOMES followed by transfection with CDX2, ETS2 and/or AP1, the expression of -654-oIFNT-Luc was decreased. Also, EOMES factor inhibited the stimulatory activity of CDX2 alone. These results suggest that when conceptuses attach to the uterine epithelium, ovine IFNT gene transcription is down-regulated by an increase of EOMES factor expression in the attached ovine trophoblast cells.</description><identifier>ISSN: 2671-4639</identifier><identifier>EISSN: 2671-4663</identifier><identifier>DOI: 10.12750/JARB.34.4.292</identifier><language>eng</language><publisher>The Korean Society of Animal Reproduction and Biotechnology</publisher><subject>interferon-tau ; jeg3 ; transcription factor ; transfection ; trophectoderm ; 축산학</subject><ispartof>한국동물생명공학회지, 2019, 34(4), , pp.292-299</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART002545355$$DAccess content in National Research Foundation of Korea (NRF)$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Min-Su</creatorcontrib><creatorcontrib>Lim, Hyun-Joo</creatorcontrib><creatorcontrib>Lee, Ji Hwan</creatorcontrib><creatorcontrib>Hur, Tae Young</creatorcontrib><creatorcontrib>Son, Jun Kyu</creatorcontrib><title>Temporal Regulation of Ovine Interferon-tau Gene by the Transcription Factor Eomesodermin in the Peri-Implantation Period</title><title>Journal of animal reproduction & biotechnology (Online)</title><description>Interferon tau (IFNT) regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, is essential for the maintenance of early pregnancy, but a definitive mechanism for its temporal transcription has not been elucidated. We and others have observed the T-box protein eomesodermin (EOMES) exhibited high mRNA expression in the ovine embryonic trophectoderm; thus, both caudal-relatedhomeobox-2 (CDX2) and EOMES coexist during the early stages of conceptus development. Objective of this study was to examine the effect of EOMES on ovine IFNT gene transcription when evaluated with CDX2, ETS2 and AP1 transcription factors implicated in the control of cell differentiation in the trophectoderm. In this study, quantitatively via reverse transcription-polymerase chain reaction (RT-PCR) analysis between ovine trophoblast cells was initially performed, finding that transcription factors CDX2 and ‘EOMES transcription factor mRNAs’ were specific to trophectoderm cells. These mRNAs were also found in days 15, 17, and 21 ovine conceptuses. Furthermore, human choriocarcinoma JEG3 cells (trophoblast cell line) were cotransfected with an ovine IFNT (-654bp)-luciferase reporter (-654-oIFNT-Luc) construct and several transcription factor expression plasmids. Cotransfection of the reporter construct with CDX2, ETS2 and AP1 increased transcription of -654-oIFNT-Luc by about 11-fold compared with transfection of the construct alone. When cells were initially transfected with EOMES followed by transfection with CDX2, ETS2 and/or AP1, the expression of -654-oIFNT-Luc was decreased. Also, EOMES factor inhibited the stimulatory activity of CDX2 alone. These results suggest that when conceptuses attach to the uterine epithelium, ovine IFNT gene transcription is down-regulated by an increase of EOMES factor expression in the attached ovine trophoblast cells.</description><subject>interferon-tau</subject><subject>jeg3</subject><subject>transcription factor</subject><subject>transfection</subject><subject>trophectoderm</subject><subject>축산학</subject><issn>2671-4639</issn><issn>2671-4663</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNo9kUtr5DAQhE1IICHJNWdd92BHj7YsH2dDHl4GEgbfRVtuz2pjW4PsLMy_X49nCTRUU1R9l0qSB8EzIYucP_7a7H5mCjLIZCkvkhupC5GC1ury-1fldXI_Tb7heV4YA0bcJMeahkOI2LMd7b96nH0YWejY-18_EqvGmWJHMYzpjF_slRavObL5N7E64ji56A9r4wXdHCJ7DgNNoaU4-JEtdwp-UPRpNRx6HOcz_uSE9i656rCf6P6_3ib1y3P99JZu31-rp802dVJxmYJpnXYcuxw5FzkvONdaN1C2XJVOm8blvFFcGS0bQkEFAPKOQLUNSSHVbfLjjB1jZz-dtwH9qvtgP6Pd7OrKahCmMGLJVudsG_CPPUQ_YDyuhdUIcW8xzt71ZEsJLQogLIWDHJTBlnemJDBSKDKwsLIzy8UwTZG6b57gdp3MniazCizYZTL1D12qiT4</recordid><startdate>20191231</startdate><enddate>20191231</enddate><creator>Kim, Min-Su</creator><creator>Lim, Hyun-Joo</creator><creator>Lee, Ji Hwan</creator><creator>Hur, Tae Young</creator><creator>Son, Jun Kyu</creator><general>The Korean Society of Animal Reproduction and Biotechnology</general><general>사단법인 한국동물생명공학회</general><scope>AAYXX</scope><scope>CITATION</scope><scope>DOA</scope><scope>ACYCR</scope></search><sort><creationdate>20191231</creationdate><title>Temporal Regulation of Ovine Interferon-tau Gene by the Transcription Factor Eomesodermin in the Peri-Implantation Period</title><author>Kim, Min-Su ; Lim, Hyun-Joo ; Lee, Ji Hwan ; Hur, Tae Young ; Son, Jun Kyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2302-48dc6c0af5a00150700666b49d039c68bc50b303862bea1e744a0fe43dbe2123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>interferon-tau</topic><topic>jeg3</topic><topic>transcription factor</topic><topic>transfection</topic><topic>trophectoderm</topic><topic>축산학</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Min-Su</creatorcontrib><creatorcontrib>Lim, Hyun-Joo</creatorcontrib><creatorcontrib>Lee, Ji Hwan</creatorcontrib><creatorcontrib>Hur, Tae Young</creatorcontrib><creatorcontrib>Son, Jun Kyu</creatorcontrib><collection>CrossRef</collection><collection>Directory of Open Access Journals</collection><collection>Korean Citation Index</collection><jtitle>Journal of animal reproduction & biotechnology (Online)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Min-Su</au><au>Lim, Hyun-Joo</au><au>Lee, Ji Hwan</au><au>Hur, Tae Young</au><au>Son, Jun Kyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Temporal Regulation of Ovine Interferon-tau Gene by the Transcription Factor Eomesodermin in the Peri-Implantation Period</atitle><jtitle>Journal of animal reproduction & biotechnology (Online)</jtitle><date>2019-12-31</date><risdate>2019</risdate><volume>34</volume><issue>4</issue><spage>292</spage><epage>299</epage><pages>292-299</pages><issn>2671-4639</issn><eissn>2671-4663</eissn><abstract>Interferon tau (IFNT) regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, is essential for the maintenance of early pregnancy, but a definitive mechanism for its temporal transcription has not been elucidated. We and others have observed the T-box protein eomesodermin (EOMES) exhibited high mRNA expression in the ovine embryonic trophectoderm; thus, both caudal-relatedhomeobox-2 (CDX2) and EOMES coexist during the early stages of conceptus development. Objective of this study was to examine the effect of EOMES on ovine IFNT gene transcription when evaluated with CDX2, ETS2 and AP1 transcription factors implicated in the control of cell differentiation in the trophectoderm. In this study, quantitatively via reverse transcription-polymerase chain reaction (RT-PCR) analysis between ovine trophoblast cells was initially performed, finding that transcription factors CDX2 and ‘EOMES transcription factor mRNAs’ were specific to trophectoderm cells. These mRNAs were also found in days 15, 17, and 21 ovine conceptuses. Furthermore, human choriocarcinoma JEG3 cells (trophoblast cell line) were cotransfected with an ovine IFNT (-654bp)-luciferase reporter (-654-oIFNT-Luc) construct and several transcription factor expression plasmids. Cotransfection of the reporter construct with CDX2, ETS2 and AP1 increased transcription of -654-oIFNT-Luc by about 11-fold compared with transfection of the construct alone. When cells were initially transfected with EOMES followed by transfection with CDX2, ETS2 and/or AP1, the expression of -654-oIFNT-Luc was decreased. Also, EOMES factor inhibited the stimulatory activity of CDX2 alone. These results suggest that when conceptuses attach to the uterine epithelium, ovine IFNT gene transcription is down-regulated by an increase of EOMES factor expression in the attached ovine trophoblast cells.</abstract><pub>The Korean Society of Animal Reproduction and Biotechnology</pub><doi>10.12750/JARB.34.4.292</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | interferon-tau jeg3 transcription factor transfection trophectoderm 축산학 |
title | Temporal Regulation of Ovine Interferon-tau Gene by the Transcription Factor Eomesodermin in the Peri-Implantation Period |
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