Cytological, genetic, and proteomic analysis of a sesame (Sesamum indicum L.) mutant Siyl-1 with yellow–green leaf color

• Published in: Genes & genomics 2020, 42(1), , pp.25-39
• Main Authors: Gao, Tong-Mei, Wei, Shuang-Ling, Chen, Jing, Wu, Yin, Li, Feng, Wei, Li-Bin, Li, Chun, Zeng, Yan-Juan, Tian, Yuan, Wang, Dong-Yong, Zhang, Hai-Yang
• Format: Article
• Language: English
• Subjects: Animal Genetics and Genomics; Biomedical and Life Sciences; Chlorophyll; Chloroplasts; Color; Energy metabolism; Gel electrophoresis; Genetic analysis; Genotypes; Human Genetics; Leaves; Life Sciences; Microbial Genetics and Genomics; Morphology; Mutants; Mutation; Offspring; Phenotypes; Photosynthesis; Photosynthetic pigments; Pigments; Plant cells; Plant Genetics and Genomics; Research Article; Sesamum indicum; Yellow leaf; 생물학
• ISSN: 1976-9571; 2092-9293
• DOI: 10.1007/s13258-019-00876-w

Background Both photosynthetic pigments and chloroplasts in plant leaf cells play an important role in deciding on the photosynthetic capacity and efficiency in plants. Systematical investigating the regulatory mechanism of chloroplast development and chlorophyll (Chl) content variation is necessary for clarifying the photosynthesis mechanism for crops. Objective This study aims to explore the critical regulatory mechanism of leaf color mutation in a yellow–green leaf sesame mutant Siyl - 1 . Methods We performed the genetic analysis of the yellow-green leaf color mutation using the F 2 population of the mutant Siyl - 1 . We compared the morphological structure of the chloroplasts, chlorophyll content of the three genotypes of the mutant F 2 progeny. We performed the two-dimensional gel electrophoresis (2-DE) and compared the protein expression variation between the mutant progeny and the wild type. Results Genetic analysis indicated that there were 3 phenotypes of the F 2 population of the mutant Siyl - 1 , i.e., YY type with light-yellow leaf color (lethal); Yy type with yellow-green leaf color, and yy type with normal green leaf color. The yellow-green mutation was controlled by an incompletely dominant nuclear gene, Siyl - 1 . Compared with the wild genotype, the chloroplast number and the morphological structure in YY and Yy mutant lines varied evidently. The chlorophyll content also significantly decreased ( P