Enhanced salt tolerance of transgenic vegetable soybeans resulting from overexpression of a novel Δ1-pyrroline-5-carboxylate synthetase gene from Solanum torvum Swartz

• Published in: Horticulture, environment and biotechnology 2015, Environment, and Biotechnology, 56(1), , pp.94-104
• Main Authors: Zhang, Gong-Chen, Zhu, Wen-Li, Gai, Jun-Yi, Zhu, Yue-Lin, Yang, Li-Fei
• Format: Article
• Language: English
• Subjects: Agriculture; Biomedical and Life Sciences; Life Sciences; Plant Breeding/Biotechnology; Plant Ecology; Plant Physiology; Research Report; 농학
• ISSN: 2211-3452; 2211-3460
• DOI: 10.1007/s13580-015-0084-3

Vegetable soybeans [ Glycine max (L.) Merrill] are susceptible to salt stress and, thus, soil salinity can severely affect their growth and productivity. To enhance the salt tolerance of vegetable soybeans, a novel Solanum torvum Swartz Δ 1 -pyrroline-5-carboxylate synthetase gene ( StP5CS , GenBank accession number: JN606861) that encodes a critical regulatory enzyme in proline biosynthesis was transformed into the cultivar NY-1001 using an Agrobacterium -mediated transformation method. PCR and Southern blot analyses indicated that two independent T 0 fertile transgenic plants were generated. The transgenic plants transmitted the transgenes into their T 1 progeny in a 3:1 ratio. The T 2 and T 3 homozygous transgenic lines (HTLs) were examined for salt tolerance in pot and hydroponic cultures, respectively. The StP5CS overexpression conferred salt tolerance in T 2 and T 3 HTLs. Under NaCl stress conditions, the leaf scorch scores of T 2 and T 3 HTLs were significantly lower than those of wild-type (WT) plants. The plant height, leaf area, relative chlorophyll content, and number of fresh pods of T 2 and T 3 HTLs were significantly higher than those of WT plants. Compared with WT plants, T 2 and T 3 HTLs had significantly higher levels of proline and significantly lower levels of membrane lipid peroxidation. These results indicate that StP5CS overexpression in HTLs results in enhanced salt tolerance associated with higher levels of proline accumulation under salinity stress and that StP5CS can be utilized to improve salinity tolerance in vegetable crop genetic engineering.