Screening different methods of tetraploid induction in watermelon [Citrullus lanatus (thunb.) Manst. and Nakai]

• Published in: Horticulture, environment and biotechnology 2012, Environment, and Biotechnology, 53(6), , pp.521-529
• Main Authors: Noh, J.J., Watermelon Experiment Station, Jeollabuk-do Agricultural Research and Extension Services, Gochang, Republic of Korea, Sheikh, Sameena, Watermelon Experiment Station, Jeollabuk-do Agricultural Research and Extension Services, Gochang, Republic of Korea, Chon, H.G., Watermelon Experiment Station, Jeollabuk-do Agricultural Research and Extension Services, Gochang, Republic of Korea, Seong, M.H., Watermelon Experiment Station, Jeollabuk-do Agricultural Research and Extension Services, Gochang, Republic of Korea, Lim, J.H., Watermelon Experiment Station, Jeollabuk-do Agricultural Research and Extension Services, Gochang, Republic of Korea, Lee, S.G., Watermelon Experiment Station, Jeollabuk-do Agricultural Research and Extension Services, Gochang, Republic of Korea, Jung, G.T., Watermelon Experiment Station, Jeollabuk-do Agricultural Research and Extension Services, Gochang, Republic of Korea, Kim, J.M., Specialization Crop Research Institute, Jeollabuk-do Agricultural Research and Extension Services, Jinan, Republic of Korea, Ju, H.J., Chonbuk National University, Jeonju, Republic of Korea, Huh, Y.C., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea
• Format: Article
• Language: English
• Subjects: Agriculture; Biomedical and Life Sciences; chloroplast count; flow cytometer; inverted hypocotyl; Life Sciences; PASTEQUE; Plant Breeding/Biotechnology; Plant Ecology; Plant Physiology; ploidy; Research Report; SANDIA; shoot apex; WATERMELONS; 농학
• ISSN: 2211-3452; 2211-3460
• DOI: 10.1007/s13580-012-0055-x

To screen a suitable method for inducing tetraploid with high frequency, five diploid inbred lines of watermelon, WC-8C-2C, WD-2-6K, GW11, GW7, and GW2 were selected. The efficiency of chromosome doubling with three methods direct seed (DS), shoot apex (SA), and inverted hypocotyl (IH) were tested by applying two different concentrations (0.1% and 0.2%) of antimitotic agent, colchicine, during the period January, 2010 to August, 2011 at Watermelon Experiment Station, Daesan, Gochang, Jeonbuk, South Korea. Mean chloroplast counts were 12 and 19 in diploid and tetraploid, respectively. The highest rate of efficiency of generating tetraploids (29.5%) was recorded in 0.2% IH method and considered as s best method. A total of 71 new tetraploids were generated successfully using present methods and techniques. WC-8C-2C and GW7 produced maximum number of tetraploids.