Expression of the Protective Antigen for PEDV in Transgenic Duckweed, Lemna minor

• Published in: Horticulture, environment and biotechnology 2011, Environment, and Biotechnology, 52(5), , pp.511-515
• Main Authors: Ko, S.M., Subtropical Horticulture Research Institute, Jeju National University, Jeju, Republic of Korea, Sun, H.J., Subtropical Horticulture Research Institute, Jeju National University, Jeju, Republic of Korea, Oh, M.J., Biological Resource Center, KRIBB, Daejeon, Republic of Korea, Song, I.J., Subtropical Horticulture Research Institute, Jeju National University, Jeju, Republic of Korea, Kim, M.J., Subtropical Horticulture Research Institute, Jeju National University, Jeju, Republic of Korea, Sin, H.S., Subtropical Horticulture Research Institute, Jeju National University, Jeju, Republic of Korea, Goh, C.H., Research Institute for Basic Sciences, Jeju National University, Jeju, Republic of Korea, Kim, Y.W., Subtropical Horticulture Research Institute, Jeju National University, Jeju, Republic of Korea, Lim, P.O., Subtropical Horticulture Research Institute, Jeju National University, Jeju, Republic of Korea, Lee, H.Y., Subtropical Horticulture Research Institute, Jeju National University, Jeju, Republic of Korea, Kim, S.W., Biological Resource Center, KRIBB, Daejeon, Republic of Korea
• Format: Article
• Language: English
• Subjects: Agriculture; Animal diseases; Antigens; AQUATIC PLANTS; Biomedical and Life Sciences; Cultivation; Diarrhea; Duckweed; Epidemics; Feasibility studies; Floating plants; Gene expression; Infectious diseases; Lemna; Lemna minor; Life Sciences; Plant Breeding/Biotechnology; Plant Ecology; Plant Physiology; PLANTAS ACUATICAS; PLANTE AQUATIQUE; Polymerase chain reaction; porcine diarrhea; Protective antigen; Proteins; Research Report; Spike protein; transformation; Transgenic plants; Transmissible gastroenteritis; VACCIN; VACCINES; VACUNA; Viruses; 농학
• ISSN: 2211-3452; 2211-3460
• DOI: 10.1007/s13580-011-0007-x

Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1 using duckweed, Lemna minor. Stably transformed Lemna were obtained by co-cultivation with A. tumefaciens EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic Lemna, respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.