Callus Culture and Plant Regeneration from Seedling Explants in 'Poshita' Indian Ginseng

The study was conducted to establish callus culture and plant regeneration in Withania somnifera (L.) Dunal. cv. Poshita with different explants and different auxin types and concentrations. Nodal explant showed better callusing response irrespective of auxin concentrations supplemented in the media...

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Published in:Horticulture, environment and biotechnology 2011, Environment, and Biotechnology, 52(1), , pp.83-88
Main Authors: Waman, Ajit Arun, University of Agricultural Sciences, GKVK, Bengaluru, India, Konana, Umesha, University of Agricultural Sciences, GKVK, Bengaluru, India, Narayanappa, Sathyanarayana Bangalore, University of Agricultural Sciences, GKVK, Bengaluru, India, Tholakalabavi, Ashok, University of Agricultural Sciences, GKVK, Bengaluru, India, Gowda, Balakrishna, University of Agricultural Sciences, GKVK, Bengaluru, India
Format: Article
Language:English
Subjects:
Agriculture
ashwagandha
Biomedical and Life Sciences
DRUG PLANTS
IN VITRO
Life Sciences
Plant Breeding/Biotechnology
Plant Ecology
PLANT GROWTH SUBSTANCES
Plant Physiology
PLANTAS MEDICINALES
PLANTE MEDICINALE
Research Report
SUBSTANCE DE CROISSANCE VEGETALE
SUSTANCIAS DE CRECIMIENTO VEGETAL
WITHANIA SOMNIFERA
농학
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Summary:The study was conducted to establish callus culture and plant regeneration in Withania somnifera (L.) Dunal. cv. Poshita with different explants and different auxin types and concentrations. Nodal explant showed better callusing response irrespective of auxin concentrations supplemented in the media, as compared to epicotyls, hypocotyls, leaves, and root tips. Maximum callusing score of 4.28 was recorded when nodes were cultured on Murashige and Skoog's (MS) media containing 2 mgㆍL-¹ naphthaleneacetic acid (NAA). Root tip and hypocotyls explants, which were found poor in inducing callus, showed highest proliferation of 6.57 and 6.00, respectively, on media with 2,4-Dichlorophenoxyacetic acid (2,4-D) for subculture. Highest number of shoots (16.0) per explant was obtained from epicotyls and nodes cultured on 1 and 2 mgㆍL-¹ benzyladenine (BA), respectively. Indole-3-butyric acid (IBA) was found to be superior source of auxin for all roof lug parameters, except percent rooting. Number of primary roots was found maximum (24.8) with 2.0 mgㆍL-¹ IBA. Irrespective of auxin used, the length of primary root decreased with increase in concentration, maximum (6.18 cm) being in media with 0.5 mgㆍL-¹ IBA. Plantlets were best hardened when agropeat was used as a substrate.
ISSN:2211-3452
2211-3460
DOI:10.1007/s13580-011-0094-8