Loading…

Isolation and analysis of natural compounds from silkworm pupae and effect of its extracts on alcohol detoxification

Silkworm pupae have much potential and many applications as a natural medicine to promote human health. However, their chemical components have not been fully characterized or understood. HPLC analysis was conducted to determine the content ratio (%) of individual amino acids in total protein of the...

Full description

Saved in:
Bibliographic Details
Published in:Entomological research 2012, 42(1), , pp.55-62
Main Authors: Kwon, M.G., Wonkwang University, Iksan, Republic of Korea, Kim, D.S., Wonkwang University, Iksan, Republic of Korea, Lee, J.H., Wonkwang University, Iksan, Republic of Korea, Park, S.W., Wonkwang University, Iksan, Republic of Korea, Choo, Y.K., Wonkwang University, Iksan, Republic of Korea, Han, Y.S., Chonnam National University, Gwangju, Republic of Korea, Kim, J.S., Korea Food Research Institute, Sungnam, Republic of Korea, Hwang, K.A., National Academy of Agricultural Science, RDA, Suwon, Republic of Korea, Ko, K.N., Konkuk University, Seoul, Republic of Korea, Ko, K.S., Wonkwang University, Iksan, Republic of Korea
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Silkworm pupae have much potential and many applications as a natural medicine to promote human health. However, their chemical components have not been fully characterized or understood. HPLC analysis was conducted to determine the content ratio (%) of individual amino acids in total protein of the pupae. It showed that glutamic acid (18.3%), histidine (14.6%) and alanine (10.2%) are the most common amino acids in silkworm pupae. Fatty acid composition of silkworm pupae oil was revealed by high-pressure liquid chromatography and gas chromatography - mass spectroscopy analyses. They contain a high ratio of essential fatty acids, [α-linolenic acid (ω-3 fatty acid]+ linoleic acid) (49.0%), and also contain non-essential fatty acids, oleic acid (19.9%), palmitoleic acid (2.5%), palmitic acid (19.7%), stearic acid (8.6%), and eicosapentaenoic acid (EPA) (0.3%). In addition, they also contain antioxidants, quercetin diglucoside and nutritionally important riboflavin (vitamin B2). This study suggests that silkworm pupae are a nutritionally valuable food product and are applicable as cosmetic components with essential amino acids, essential fatty acids, antioxidants and vitamins. The animal experiment showed that alcohol dehydrogenase (ADH) activity was significantly higher in the liver of mice orally administered with 0.5 mg/mL of silkworm extract and alcohol than with commercial Dawn808∨TM and alcohol, indicating that silkworm pupae extracts have alcohol detoxification activity.
ISSN:1738-2297
1748-5967
1748-5967
DOI:10.1111/j.1748-5967.2011.00439.x