Molecular analysis of mitochondrial cytochrome oxidase I gene of Aedes aegypti L. mosquitoes

• Published in: Journal of Asia-Pacific entomology 2020, 23(1), , pp.51-59
• Main Authors: Gandhi, Ratnapal, Yadav, Kamlesh K., Patil, Prabhakargouda B., Bihani, Pankaj, Char, Bharat, Dasgupta, Shaibal K., Zehr, Usha B., Barwale, Shirish R.
• Format: Article
• Language: English
• Subjects: Aedes aegypti; Cytochrome C oxidase-I; Haplotype; OX513A; Phylogenetic; 농학
• ISSN: 1226-8615; 1876-7990
• DOI: 10.1016/j.aspen.2019.10.006

[Display omitted] •COI of 9 field samples and 3 laboratory strain including transgenic were analyzed.•Analysis of COI using phylogenetic, distance matrix, MSA & nucleotide frequency.•Haplotype analysis revealed presence of 5 haplotypes.•Transgenic OX513A strain showed similarity with Indian and worldwide isolates. Aedes aegypti is the most important arboviral vector worldwide. Recent studies reported that genetic variations and gene flow among same mosquito species is responsible for different disease transmission rate. Hence, to understand the relationship between genetic diversity and disease transmission potential, study on genetic variations among mosquito populations is essential. The aim of present study was to investigate the genetic variations of Ae. aegypti targeting COI gene from nine villages of Jalna District, Maharashtra and three laboratory strains originated from Aurangabad, Delhi and transgenic OX513A strain imported from OXITEC, UK. OX513A strain consists of a self-limiting dominant lethal gene construct intended for its use in suppression of Ae. aegyptipopulation by sustained male adult releases in the environment. Mosquito eggs from field and laboratory strains were reared to adults and identified on the basis of morphological characteristics followed by COI gene sequence. Result of MSA and haplotype analysis revealed low genetic variations among field samples and Aurangabad strain, belonged to two haplotypes (H1 and H2) except Ramkheda village represented by separate haplotype H3. Other laboratory DEL strain and transgenic OX513A have great genetic variability to all isolates and have a separate haplotypes H4 and H5. Similar results were observed in phylogenetic analysis. Our observation of phylogenies revealed close relationship among the DEL and transgenic strain OX513A with few Indian and worldwide isolates. The information on genetic variability of mosquito population could help to understand and design the strategies for risk mitigation and effective implementation of new vector control tools like genetically modified mosquitoes.