A gammaherpesvirus establishes persistent infection in neuroblastoma cells

Gammaherpesvirus (γHV) infection of the central nervous system (CNS) has been implicated in diverse neurological diseases, and murine γHV-68 (MHV-68) is known to persist in the brain after cerebral infection. The underlying molecular mechanisms of persistency of virus in the brain are poorly underst...

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Bibliographic Details
Published in:Molecules and cells 2014, 37(7), , pp.518-525
Main Authors: Cho, Hye-Jeong, Song, Moon Jung
Format: Article
Language:English
Subjects:
Animals
Brain - pathology
Brain - virology
Butyric Acid - pharmacology
Cell Line, Tumor
Cell Proliferation
Gammaherpesvirinae - drug effects
Gammaherpesvirinae - physiology
Ganciclovir - pharmacology
Genetic Vectors
Herpesviridae Infections - drug therapy
Herpesviridae Infections - physiopathology
Humans
Mice
Neuroblastoma - drug therapy
Neuroblastoma - pathology
Neuroblastoma - virology
Pyridines - pharmacology
Virus Latency - drug effects
Virus Replication - drug effects
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Summary:Gammaherpesvirus (γHV) infection of the central nervous system (CNS) has been implicated in diverse neurological diseases, and murine γHV-68 (MHV-68) is known to persist in the brain after cerebral infection. The underlying molecular mechanisms of persistency of virus in the brain are poorly understood. Here, we characterized a unique pattern of MHV-68 persistent infection in neuroblastoma cells. On infection with MHV-68, both murine and human neuroblastoma cells expressed viral lytic proteins and produced virions. However, the infected cells survived productive infection and could be cultured for multiple passages without affecting their cellular growth. Latent infection as well as productive replication was established in these prolonged cultures, and lytic replication was further increased by treatment with lytic inducers. Our results provide a novel system to study persistent infection of γHVs in vitro following de novo infection and suggest application of MHV-68 as a potential gene transfer vector to the brain.
ISSN:1016-8478
0219-1032
DOI:10.14348/molcells.2014.0024