Requirement of vesicle-associated membrane protein 721 and 722 for sustained growth during immune responses in Arabidopsis

• Published in: Molecules and cells 2013, 35(6), , pp.481-488
• Main Authors: Yun, H.S., Max-Planck-Institut fur Zuchtungsforschung, Germany, Kwaaitall, M., Max-Planck-Institut fur Zuchtungsforschung, Germany, Kato, N., Louisiana State University, LA, USA, Yi, C.H., Dankook University, Yongin, Republic of Korea, Park, S.H., Dankook University, Yongin, Republic of Korea, Sato, M.H., Kyoto Prefectural University, Kyoto, Japan, Schulze-Lefert, P., Max-Planck-Institut fur Zuchtungsforschung, Germany, Kwon, C.A., Max-Planck-Institut fur Zuchtungsforschung, Germany
• Format: Article
• Language: English
• Subjects: Arabidopsis - immunology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - immunology; Arabidopsis Proteins - metabolism; Biochemistry; Biomedical and Life Sciences; Biomedicine; Biotechnology; Cell Biology; Cell Growth Processes - genetics; Cell Growth Processes - immunology; Cell Membrane - metabolism; Cells, Cultured; Gene Dosage - genetics; Immunity - genetics; Life Sciences; plant growth,plant immune responses,PM syntaxins,VAMP721/722; Proteasome Endopeptidase Complex - metabolism; Protein Transport - genetics; Protein Transport - immunology; Qa-SNARE Proteins - metabolism; Qb-SNARE Proteins - metabolism; Qc-SNARE Proteins - metabolism; R-SNARE Proteins - genetics; R-SNARE Proteins - immunology; R-SNARE Proteins - metabolism; Research Article; SECRECION; SECRETION; Seedlings - genetics; 생물학
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1007/s10059-013-2130-2

Extracellular immune responses to ascomycete and oomycete pathogens in Arabidopsis are dependent on vesicle-associated secretion mediated by the SNARE proteins PEN1 syntaxin, SNAP33 and endomembrane-resident VAMP721/722. Continuous movement of functional GFP-VAMP722 to and from the plasma membrane in non-stimulated cells reflects the second proposed function of VAMP721/722 in constitutive secretion during plant growth and development. Application of the bacterium-derived elicitor flg22 stabilizes VAMP721/722 that are otherwise constitutively degraded via the 26S proteasome pathway. Depletion of VAMP721/722 levels by reducing VAMP721/722 gene dosage enhances flg22-induced seedling growth inhibition in spite of elevated VAMP721/722 abundance. We therefore propose that plants prioritize the deployment of the corresponding secretory pathway for defense over plant growth. Interstingly, VAMP721/722 specifically interact in vitro and in vivo with the plasma membrane syntaxin SYP132 that is required for plant growth and resistance to bacteria. This suggests that the plant growth/immunity-involved VAMP721/722 form SNARE complexes with multiple plasma membrane syntaxins to discharge cue-dependent cargo molecules.