Dynamic contrast-enhanced MRI for monitoring antiangiogenic treatment: determination of accurate and reliable perfusion parameters in a longitudinal study of a mouse xenograft model

• Published in: Korean journal of radiology 2013, 14(4), , pp.589-596
• Main Authors: Song, Youngkyu, Cho, Gyunggoo, Suh, Ji-Yeon, Lee, Chang Kyung, Kim, Young Ro, Kim, Yoon Jae, Kim, Jeong Kon
• Format: Article
• Language: English
• Subjects: Angiogenesis Inhibitors - therapeutic use; Animals; Contrast Media; Data analysis; Experimental and Others; Female; Heterografts; Indoles - therapeutic use; Longitudinal Studies; Magnetic Resonance Imaging - methods; Mice; Mice, Inbred BALB C; Neoplasm Transplantation; Neoplasms, Experimental - diagnosis; Neoplasms, Experimental - drug therapy; Neoplasms, Experimental - pathology; Pyrroles - therapeutic use; Reproducibility of Results; Tumor Burden; Tumors; 방사선과학
• ISSN: 1229-6929; 2005-8330
• DOI: 10.3348/kjr.2013.14.4.589

To determine the reliable perfusion parameters in dynamic contrast-enhanced MRI (DCE-MRI) for the monitoring antiangiogenic treatment in mice. Mice, with U-118 MG tumor, were treated with either saline (n = 3) or antiangiogenic agent (sunitinib, n = 8). Before (day 0) and after (days 2, 8, 15, 25) treatment, DCE examinations using correlations of perfusion parameters (Kep, Kel, and A(H) from two compartment model; time to peak, initial slope and % enhancement from time-intensity curve analysis) were evaluated. Tumor growth rate was found to be 129% ± 28 in control group, -33% ± 11 in four mice with sunitinib-treatment (tumor regression) and 47% ± 15 in four with sunitinib-treatment (growth retardation). Kep (r = 0.80) and initial slope (r = 0.84) showed strong positive correlation to the initial tumor volume (p < 0.05). In control mice, tumor regression group and growth retardation group animals, Kep (r : 0.75, 0.78, 0.81, 0.69) and initial slope (r : 0.79, 0.65, 0.67, 0.84) showed significant correlation with tumor volume (p < 0.01). In four mice with tumor re-growth, Kep and initial slope increased 20% or greater at earlier (n = 2) than or same periods (n = 2) to when the tumor started to re-grow with 20% or greater growth rate. Kep and initial slope may a reliable parameters for monitoring the response of antiangiogenic treatment.